Triggering method in assisted reproduction alters the cumulus cell transcriptome

Autor: Julieta Caballero, Itai Gat, Brandon A. Wyse, Hanna Balakier, Noga Fuchs Weizman, Clifford Librach, Shlomit Kenigsberg, Mugundhine Sangaralingam
Rok vydání: 2019
Předmět:
Adult
0301 basic medicine
Agonist
endocrine system
Pregnancy Rate
Reproductive Techniques
Assisted
medicine.drug_class
media_common.quotation_subject
Oocyte Retrieval
Fertilization in Vitro
Biology
Cumulus Cell
Hormone antagonist
Chorionic Gonadotropin
Polymerase Chain Reaction
Antioxidants
Andrology
Transcriptome
Ovarian Hyperstimulation Syndrome
03 medical and health sciences
Oogenesis
0302 clinical medicine
Ovarian Follicle
Ovulation Induction
Pregnancy
medicine
Humans
Ovarian reserve
media_common
Cumulus Cells
030219 obstetrics & reproductive medicine
Ovary
Obstetrics and Gynecology
Oocyte
Extracellular Matrix
030104 developmental biology
medicine.anatomical_structure
Reproductive Medicine
Case-Control Studies
Oocytes
Female
Reproduction
hormones
hormone substitutes
and hormone antagonists
Developmental Biology
Hormone
Zdroj: Reproductive BioMedicine Online. 39:211-224
ISSN: 1472-6483
DOI: 10.1016/j.rbmo.2019.03.213
Popis: Research question How does the choice of triggering final oocyte maturation affect the cumulus cell transcriptome? Design Sixty patients undergoing gonadotrophin-releasing hormone antagonist (GnRH-ant) IVF cycles were recruited for this nested case–control study. Patients were stratified into three subgroups based on their ovarian reserve (high, normal and low). Triggering final oocyte maturation was accomplished by either single trigger (with human chorionic gonadotrophin [HCG] only or gonadotrophin-releasing hormone agonist [GnRH-ag] only) or dual trigger combining HCG and GnRH-ag. The choice of trigger was at the discretion of the treating physician. Within each group patients receiving a dual trigger were matched by demographic and pre-stimulation parameters with patients receiving a single trigger. The matching was performed to minimize the biological variability within each subgroup. Thirty patients were included in the final analysis. Cumulus cells were stripped away from the retrieved oocytes. Cumulus cells from three sibling oocytes were pooled, the RNA extracted and libraries prepared. Next-generation sequencing was performed on all samples. Results Dual triggering supports key ovarian pathways of oocyte maturation and extracellular matrix remodelling, while attenuating vasculo-endothelial growth and providing antioxidant protection to the growing follicles. Conclusions This is the first study to delineate key transcriptomic changes under dual triggering of final oocyte maturation, across different patient populations. The findings underline the need for larger-scale studies validating transcriptomic effects of methods for triggering final oocyte maturation. Furthermore, there is a need for large-scale clinical randomized controlled studies to relate the findings of this study with clinical outcomes.
Databáze: OpenAIRE
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