Fabricating Nanoscale DNA Patterns with Gold Nanowires
| Autor: | Aaron R. Halpern, Yulin Chen, Sheng-Chin Kung, David K. Taggart, Reginald M. Penner, Robert M. Corn |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
chemistry.chemical_classification
Nanowires Polymers Chemistry viruses genetic processes Nanowire DNA Single-Stranded Nucleic Acid Hybridization Nanoparticle Nanotechnology Substrate (electronics) Polymer Microscopy Atomic Force environment and public health Article Analytical Chemistry enzymes and coenzymes (carbohydrates) Microscopy Fluorescence Colloidal gold Monolayer health occupations Gold Biosensor Nanoscopic scale |
| Zdroj: | Analytical Chemistry. 82:3365-3370 |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | Surface patterns of single-stranded DNA (ssDNA) consisting of nanoscale lines as thin as 40 nm were fabricated on polymer substrates for nanotechnology and bioaffinity sensing applications. Large scale arrays (with areas up to 4 cm(2)) of ssDNA "nanolines" were created on streptavidin-coated polymer (PDMS) surfaces by transferring biotinylated ssDNA from a master pattern of gold nanowires attached to a glass substrate. The gold nano-wires were first formed on the glass substrate by the process of lithographically patterned nanowire electrodeposition (LPNE), and then "inked" with biotinylated ssDNA by hybridization adsorption to a thiol-modified ssDNA monolayer attached to the gold nanowires. The transferred ssDNA nanolines were capable of hybridizing with ssDNA from solution to form double-stranded DNA (dsDNA) patterns; a combination of fluorescence and atomic force microscopy (AFM) measurements were used to characterize the dsDNA nanoline arrays. To demonstrate the utility of these surfaces for biosensing, optical diffraction measurements of the hybridization adsorption of DNA-coated gold nanoparticles onto the ssDNA nanoline arrays were used to detect a specific target sequence of unlabeled ssDNA in solution. |
| Databáze: | OpenAIRE |
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