Binder of Sperm Proteins protect ram spermatozoa from freeze-thaw damage

Autor: Xavier Druart, Valérie Labas, Simon P. de Graaf, Kiri Farmer, Ana Paula Teixeira-Gomes, Guillaume Tsikis, Taylor Pini, T. Leahy
Přispěvatelé: University of Sydney, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA), Australian Wool Innovation [ON_00252], NSW Stud Merino Breeders Association, European Regional Development Fund [3069], Conseil Regional du Centre, French National Institute for Agricultural Research, French National Institute of Health and Medical Research (INSERM), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2018
Předmět:
Zdroj: Cryobiology
Cryobiology, Elsevier, 2018, 82, pp.78-87. ⟨10.1016/j.cryobiol.2018.04.005⟩
ISSN: 0011-2240
1090-2392
DOI: 10.1016/j.cryobiol.2018.04.005
Popis: International audience; Cryopreservation causes sub-lethal damage which limits the fertility of frozen thawed spermatozoa. Seminal plasma has been investigated as a cryoprotectant, but has yielded inconsistent results due to considerable variation in its constituents. Individual seminal plasma proteins offer an ideal alternative to whole seminal plasma, and several have been correlated with freezing success. Binder of Sperm Proteins (BSPs) are abundant ram seminal plasma proteins which have been suggested to have significant protective effects on ram spermatozoa during cold shock. This is in direct opposition to bull spermatozoa, where BSPs cause sperm deterioration during in vitro handling. We investigated the potential of BSP1 and BSP5 to prevent freezing associated damage to important functional parameters of ram spermatozoa. BSPs purified by size exclusion chromatography improved post thaw motility and penetration through artificial mucus. Highly purified BSP1 and BSP5, isolated by gelatin affinity and RP-HPLC, improved motility and membrane integrity, and reduced post thaw protein tyrosine phosphorylation. Exposure to BSP5 before freezing increased the amount of phosphatidylethanolamine on the sperm surface after thawing. Neither BSP1 nor BSP5 prevented freezing associated changes in membrane lipid disorder. These results suggest that BSPs may significantly improve freezing outcomes of ram spermatozoa.
Databáze: OpenAIRE
Externí odkaz: