Lactobacillus delbrueckii as a potential skin adjuvant for induction of type 1 immune responses
| Autor: | Leda Quercia Vieira, Olindo Assis Martins-Filho, Afonso Lc, Castro Jm, Nicoli, Castanheira Lg |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Male medicine.medical_treatment T-Lymphocytes Peripheral blood mononuclear cell Proinflammatory cytokine law.invention Probiotic Immune system Adjuvants Immunologic law Lactobacillus medicine Macrophage Humans Cells Cultured Lactobacillus delbrueckii biology Vaccination food and beverages Cell Differentiation Macrophage Activation Middle Aged Th1 Cells biology.organism_classification In vitro Immunology Leukocytes Mononuclear Cytokines Female Adjuvant |
| Zdroj: | Frontiers in bioscience : a journal and virtual library. 12 |
| ISSN: | 1093-9946 |
| Popis: | We evaluated the ability of Lactobacillus delbrueckii UFV H2b20, a probiotic candidate, to stimulate the production of inflammatory cytokines and to induce macrophage activation and Th1 differentiation in peripheral blood mononuclear cells (PBMC) from healthy volunteers. Our results show that PBMC stimulated with heat-killed Lact. delbrueckii produced elevated levels of IL-12, IFN-gamma and TNF-alpha but no IL-10. IFN-gamma production was IL-12 dependent with NK cells as the main source. Furthermore, PBMC infected with Leishmania amazonensis presented elevated microbicidal activity when co-incubated with Lact. delbrueckii. Finally, Lact. delbrueckii was capable of inducing in vitro differentiation of L. amazonensis-specific Th1 cells. These findings suggest that this probiotic may be used as an adjuvant in vaccination protocols. |
| Databáze: | OpenAIRE |
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