Vascular architecture in free flaps: Analysis of vessel morphology and morphometry in murine free flaps
Autor: | Maximilian Krüger, Lukas Jäger, Maximilian Ackermann, Andreas Pabst, Peer W. Kämmerer |
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Rok vydání: | 2017 |
Předmět: |
CD31
Male Time Factors medicine.medical_treatment Femoral vein Neovascularization Physiologic Femoral artery Free flap Revascularization Corrosion Casting Biochemistry Free Tissue Flaps Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine medicine.artery medicine Animals Intussusceptive angiogenesis Microvessel Skin Groin business.industry Anastomosis Surgical 030206 dentistry Cell Biology Anatomy Femoral Vein Femoral Artery Platelet Endothelial Cell Adhesion Molecule-1 medicine.anatomical_structure 030220 oncology & carcinogenesis Microvessels Models Animal cardiovascular system Cardiology and Cardiovascular Medicine business Biomarkers |
Zdroj: | Microvascular research. 118 |
ISSN: | 1095-9319 |
Popis: | The aim of this study was to analyze the development of vascular architecture as well as vascular morphometry and morphology of anastomosed microvascular free flaps. Free pectoral skin flaps were raised in 25 rats and anastomosed to the femoral vessels in the groin region. CD31 immunohistology was performed after 3, 7 and 12 d (each 5 animals each) to analyze microvessel density (MVD), microvessel area (MVA) and microvessel size (MVS). Microvascular corrosion casting was performed after 7 and 12 d (5 animals each) to analyze vessel diameter (VD), intervascular distance (IVD), interbranching distance (IBD), and branching angle (BA). Further on, sprout and pillar density as hallmarks of sprouting and intussusceptive angiogenesis were analyzed. Pectoral skin isles from the contralateral side served as controls. A significantly increased MVD was found after 7 and 12 d (p each0.001). MVA was significantly increased after 3, 7 and 12 d (p each0.001) and a significantly increased MVS was analyzed after 3 and 7 d (p each0.001). VD and IVD were significantly increased after 7 and 12 d (p each0.001). For IBD, a significantly increase was measured after 7 d (p 0.001). For IBA, sprout and pillar density, no significant differences were found (p each ≥0.05). Significant changes in the vascular architecture of free flaps after successful microvascular anastomosis were seen. Since there was no evidence for sprout and pillar formation within the free flaps, the increased MVD and flap revascularization might be induced by the receiving site. |
Databáze: | OpenAIRE |
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