Differential effects of serum constituents on apoptosis induced by the cyclopentenone prostaglandin 15-deoxy-Δ12,14-prostaglandin J2 in WISH epithelial cells
| Autor: | Murray D. Mitchell, Timothy A. Sato, Elicia B.E Berry, Rachel J. A. Helliwell, R. Stewart Gilmour |
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| Rok vydání: | 2004 |
| Předmět: |
Serum
Cyclopentenone medicine.medical_specialty Programmed cell death Clinical Biochemistry Serum albumin Prostaglandin Apoptosis Cell Line Receptor IGF Type 1 chemistry.chemical_compound Neoplasms Internal medicine medicine Humans Serum Albumin Cyclopentenone prostaglandins Inflammation biology Prostaglandin D2 Albumin Epithelial Cells Cell Biology Endocrinology chemistry biology.protein Intercellular Signaling Peptides and Proteins lipids (amino acids peptides and proteins) Signal transduction Signal Transduction |
| Zdroj: | Prostaglandins, Leukotrienes and Essential Fatty Acids. 71:191-197 |
| ISSN: | 0952-3278 |
| DOI: | 10.1016/j.plefa.2004.04.001 |
| Popis: | Cyclopentenone prostaglandins, Delta(12)-PGJ(2) and 15d-PGJ(2), have potent anti-tumour and anti-inflammatory activities, and have been shown to induce apoptosis in amnion-derived WISH cells. In this study, we have investigated the protective effects of serum and its constituents (growth factors and albumin) on Delta(12) -PGJ(2) and 15d-PGJ(2)-induced apoptosis in WISH cells. Serum (0.5% w/v) was protective against both A 12 -PGJ(2) and 15d-PGJ2-induced apoptosis. This was not due to the presence of serum-derived growth factors (EGF, IGF-1 and IGF-2), since they had no significant effect on 15d-PGJ(2)-induced cell death. In contrast, IGF-1 partially inhibited etoposide-induced apoptosis, confirming the presence of a functional IGF-1 receptor signalling system. Albumin was identified as the key survival factor in serum, since albumin and delipidated albumin exhibited the same level of protection from 15d-PGJ(2)-induced apoptosis as serum itself. The potential for serum albumin to regulate the bioactivity of cyclopentenone PGs may be of considerable importance in pathological conditions where roles for cyclopentenone PGs have been identified. (C) 2004 Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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