An alternative D. melanogaster 7SK snRNP
| Autor: | David H. Price, Nicolas Buisine, Patricia Uguen, Duy Nguyen, Olivier Fayol, Annemieke A. Michels, Olivier Bensaude |
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| Rok vydání: | 2021 |
| Předmět: |
RNA polymerase II
Biology P-TEFb Transcription (biology) RNA Small Nuclear 7SK RNA Animals Drosophila Proteins Positive Transcriptional Elongation Factor B snRNP Molecular Biology QH573-671 Cyclin T Research RNA-Binding Proteins RNA 7SK Small Nuclear RNA Cell Biology Ribonucleoproteins Small Nuclear Non-coding RNA 7SK snRNA Cell biology Drosophila melanogaster Ribonucleoproteins Long non-coding RNA biology.protein RNA Long Noncoding Drosophila Cytology Small nuclear RNA Protein Binding Transcription Factors |
| Zdroj: | BMC Molecular and Cell Biology, Vol 22, Iss 1, Pp 1-12 (2021) BMC Molecular and Cell Biology |
| ISSN: | 2661-8850 |
| DOI: | 10.1186/s12860-021-00381-7 |
| Popis: | Background The 7SK small nuclear RNA (snRNA) found in most metazoans is a key regulator of P-TEFb which in turn regulates RNA polymerase II elongation. Although its primary sequence varies in protostomes, its secondary structure and function are conserved across evolutionary distant taxa. Results Here, we describe a novel ncRNA sharing many features characteristic of 7SK RNAs, in D. melanogaster. We examined the structure of the corresponding gene and determined the expression profiles of the encoded RNA, called snRNA:7SK:94F, during development. It is probably produced from the transcription of a lncRNA which is processed into a mature snRNA. We also addressed its biological function and we show that, like dm7SK, this alternative 7SK interacts in vivo with the different partners of the P-TEFb complex, i.e. HEXIM, LARP7 and Cyclin T. This novel RNA is widely expressed across tissues. Conclusion We propose that two distinct 7SK genes might contribute to the formation of the 7SK snRNP complex in D. melanogaster. |
| Databáze: | OpenAIRE |
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