Metagenomic next-generation sequencing as a diagnostic tool for toxoplasmic encephalitis
Autor: | Chunhua Xu, Wei Chen, Yang Lin, Cong Cheng, Zhiliang Hu, Xing Weng, Hongxia Wei |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
Adult
0301 basic medicine Microbiology (medical) Toxoplasmic encephalitis medicine.medical_specialty 030106 microbiology lcsh:QR1-502 Toxoplasma gondii Case Report Genome DNA sequencing lcsh:Microbiology law.invention lcsh:Infectious and parasitic diseases 03 medical and health sciences symbols.namesake 0302 clinical medicine Medical microbiology law parasitic diseases medicine Humans lcsh:RC109-216 030212 general & internal medicine Polymerase chain reaction Sanger sequencing biology Diffuse brain lesions Human immunodeficiency virus lcsh:RM1-950 High-Throughput Nucleotide Sequencing General Medicine medicine.disease biology.organism_classification Virology Infectious Diseases lcsh:Therapeutics. Pharmacology Metagenomics Toxoplasmosis Cerebral symbols Next-generation sequencing Encephalitis Genome Protozoan Toxoplasma |
Zdroj: | Annals of Clinical Microbiology and Antimicrobials, Vol 17, Iss 1, Pp 1-6 (2018) Annals of Clinical Microbiology and Antimicrobials |
ISSN: | 1476-0711 |
Popis: | Background More than 100 different pathogens can cause encephalitis. Testing of all the neurological pathogens by conventional methods can be difficult. Metagenomic next-generation sequencing (NGS) could identify the infectious agents in a target-independent manner. The role of this novel method in clinical diagnostic microbiology still needs to be evaluated. In present study, we used metagenomic NGS to search for an infectious etiology in a human immunodeficiency virus (HIV)-infected patient with lethally diffuse brain lesions. Sequences mapping to Toxoplasma gondii were unexpectedly detected. Case presentation A 31-year-old HIV-infected patient presented to hospital in a critical ill condition with a Glasgow coma scale score of 3. Brain magnetic resonance imaging showed diffuse brain abnormalities with contrast enhancement. Metagenomic NGS was performed on DNA extract from 300 μL patient’s cerebrospinal fluid (CSF) with the BGISEQ-50 platform. The sequencing detection identified 65,357 sequence reads uniquely aligned to the Toxoplasma gondii genome. Presence of Toxoplasma gondii genome in CSF was further verified by Toxoplasma gondii-specific polymerase chain reaction and Sanger sequencing. Altogether, those results confirmed the diagnosis of toxoplasmic encephalitis. Conclusions This study suggests that metagenomic NGS may be a useful diagnostic tool for toxoplasmic encephalitis. As metagenomic NGS is able to identify all pathogens in a single run, it may be a promising strategy to explore the clinical causative pathogens in central nervous system infections with atypical features. |
Databáze: | OpenAIRE |
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