Metagenomic next-generation sequencing as a diagnostic tool for toxoplasmic encephalitis

Autor: Chunhua Xu, Wei Chen, Yang Lin, Cong Cheng, Zhiliang Hu, Xing Weng, Hongxia Wei
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Adult
0301 basic medicine
Microbiology (medical)
Toxoplasmic encephalitis
medicine.medical_specialty
030106 microbiology
lcsh:QR1-502
Toxoplasma gondii
Case Report
Genome
DNA sequencing
lcsh:Microbiology
law.invention
lcsh:Infectious and parasitic diseases
03 medical and health sciences
symbols.namesake
0302 clinical medicine
Medical microbiology
law
parasitic diseases
medicine
Humans
lcsh:RC109-216
030212 general & internal medicine
Polymerase chain reaction
Sanger sequencing
biology
Diffuse brain lesions
Human immunodeficiency virus
lcsh:RM1-950
High-Throughput Nucleotide Sequencing
General Medicine
medicine.disease
biology.organism_classification
Virology
Infectious Diseases
lcsh:Therapeutics. Pharmacology
Metagenomics
Toxoplasmosis
Cerebral
symbols
Next-generation sequencing
Encephalitis
Genome
Protozoan
Toxoplasma
Zdroj: Annals of Clinical Microbiology and Antimicrobials, Vol 17, Iss 1, Pp 1-6 (2018)
Annals of Clinical Microbiology and Antimicrobials
ISSN: 1476-0711
Popis: Background More than 100 different pathogens can cause encephalitis. Testing of all the neurological pathogens by conventional methods can be difficult. Metagenomic next-generation sequencing (NGS) could identify the infectious agents in a target-independent manner. The role of this novel method in clinical diagnostic microbiology still needs to be evaluated. In present study, we used metagenomic NGS to search for an infectious etiology in a human immunodeficiency virus (HIV)-infected patient with lethally diffuse brain lesions. Sequences mapping to Toxoplasma gondii were unexpectedly detected. Case presentation A 31-year-old HIV-infected patient presented to hospital in a critical ill condition with a Glasgow coma scale score of 3. Brain magnetic resonance imaging showed diffuse brain abnormalities with contrast enhancement. Metagenomic NGS was performed on DNA extract from 300 μL patient’s cerebrospinal fluid (CSF) with the BGISEQ-50 platform. The sequencing detection identified 65,357 sequence reads uniquely aligned to the Toxoplasma gondii genome. Presence of Toxoplasma gondii genome in CSF was further verified by Toxoplasma gondii-specific polymerase chain reaction and Sanger sequencing. Altogether, those results confirmed the diagnosis of toxoplasmic encephalitis. Conclusions This study suggests that metagenomic NGS may be a useful diagnostic tool for toxoplasmic encephalitis. As metagenomic NGS is able to identify all pathogens in a single run, it may be a promising strategy to explore the clinical causative pathogens in central nervous system infections with atypical features.
Databáze: OpenAIRE
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