An ICAM-1 like cell adhesion molecule is responsible for CD34 positive haemopoietic stem cells adhesion to bone-marrow stroma
| Autor: | S.S. Desai, S.G.A. Rao, A. Deora, Vivek Tanavde, V.S. Chitnis |
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| Rok vydání: | 1996 |
| Předmět: |
Stromal cell
Cell adhesion molecule Immunoblotting Soluble cell adhesion molecules Antibodies Monoclonal Antigens CD34 Bone Marrow Cells Cell Biology General Medicine Biology Intercellular adhesion molecule Flow Cytometry Hematopoietic Stem Cells Intercellular Adhesion Molecule-1 Molecular biology Cell biology Culture Media Haematopoiesis Cell Adhesion Humans Neural cell adhesion molecule Stem cell Stromal Cells Cell adhesion Cells Cultured |
| Zdroj: | Cell biology international. 20(4) |
| ISSN: | 1065-6995 |
| Popis: | The microenvironment in the haematopoietic organs plays an important role in regulating and sustaining differentiation and self-renewal of haematopoietic stem cells. Although crucial for stem cell maintenance and homing, the stromal cell–stem cell interactions are poorly understood. Here we show that an ICAM-like molecule is responsible for stem cell adhesion to stromal cells in vitro . The molecule was characterized by a monoclonal antibody 3E 10 . Immunoblotting results indicated that the molecule had an electrophoretic mobility equal to that of intercellular cell adhesion molecule-1 (ICAM-1). Binding inhibition assays, however, showed that inhibition of binding of enriched CD34 cells by 3E 10 was more prominent in comparison with that of ICAM-1. |
| Databáze: | OpenAIRE |
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