A study of the small spherical high density lipoproteins of patients afflicted with familial lecithin: cholesterol acyltransferase deficiency
| Autor: | John A. Glomset, Weiling C. King, Egil Gjone, C.H. Chen, Kenneth R. Applegate, Kaare R. Norum |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1984 |
| Předmět: |
Intermediate-density lipoprotein
Very low-density lipoprotein Lecithin cholesterol acyltransferase deficiency Apolipoprotein B biology Cholesterol Reverse cholesterol transport Sterol O-acyltransferase Cell Biology QD415-436 medicine.disease Biochemistry chemistry.chemical_compound Endocrinology chemistry medicine biology.protein lipids (amino acids peptides and proteins) Lipoprotein |
| Zdroj: | Journal of Lipid Research, Vol 25, Iss 3, Pp 269-282 (1984) |
| ISSN: | 0022-2275 |
| Popis: | We studied the effects of the 1ecithin:cholesterol acyl- transferase reaction on the size and composition of the small spherical high density lipoproteins of patients afflicted with fa- milial 1ecithin:cholesterol acyltransferase deficiency. We isolated these lipoproteins by preparative ultracentrifugation and rate zonal ultracentrifugation, determined their diameter by gradient gel electrophoresis, and then calculated their composition by relating measurements of their lipid and apolipoprotein content to particle volume. Our results revealed lipoprotein particles 6.0-6.2 nm in diameter that contained approximately 2 mol- ecules of apolipoprotein A-I, 37-38 molecules of phospholipid, 3-9 molecules of unesterified cholesterol, 1-2 molecules of cho- lesteryl ester, and 1-2 molecules of triacylglycerol.being incubated with 1ecithin:cholesterol acyltransferase and a source ofadditional unesterified cholesterol, these lipoproteins increased in content of total cholesterol and in particle size to form discrete lipoprotein products 6.6-8.6 nm in diameter. The increase in size occurred despite a net decrease in product unesterified cholesterol and phospholipid and though the net change in total lipid volume was small. Moreover, specific product lipoproteins, isolated by rate zonal ultracentrifugation, contained an increased amount of apolipoprotein A-I. These results seem best explained by a process involving 1ecithin:cholesterol acyltransferase-induced particle rearrangement reactions. The possibility that a similar process normally occurs in vivo deserves to be explored."Chen, C., K. Applegate, W. C. King, J. A. Glomset, K. R. Norum, and E. Cjone. A study of the small spherical high density li- poproteins of patients afflicted with familial 1ecithin:cholesterol acyltransferase deficiency. J. Lipid Res. 1984. 25: 269-282. |
| Databáze: | OpenAIRE |
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