Evaluation of Cell Lines for the Isolation of Foot-and-Mouth Disease Virus and Other Viruses Causing Vesicular Disease
Autor: | Valérie Mioulet, Ashley R. Gray, Donald P. King, Mehreen Azhar, Britta A. Wood, Elisabeth Henry |
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Rok vydání: | 2020 |
Předmět: |
diagnosis
040301 veterinary sciences viruses Porcine kidney Disease Biology vesicular viruses Virus 0403 veterinary science 03 medical and health sciences medicine Original Research 030304 developmental biology virus isolation 0303 health sciences lcsh:Veterinary medicine General Veterinary Foot-and-mouth disease foot-and-mouth disease virus cell line 04 agricultural and veterinary sciences medicine.disease biology.organism_classification Isolation (microbiology) Virology Sensitive cell Cell culture lcsh:SF600-1100 Veterinary Science Foot-and-mouth disease virus |
Zdroj: | Frontiers in Veterinary Science Frontiers in Veterinary Science, Vol 7 (2020) |
ISSN: | 2297-1769 |
Popis: | The most sensitive cell culture system for the isolation of foot-and-mouth disease virus (FMDV) is primary bovine thyroid (BTY) cells. However, BTY cells are seldom used because of the challenges associated with sourcing thyroids from FMDV-negative calves (particularly in FMD endemic countries), and the costs and time required to regularly prepare batches of cells. Two continuous cell lines, a fetal goat tongue cell line (ZZ-R 127) and a fetal porcine kidney cell line (LFBK-αVβ6), have been shown to be highly sensitive to FMDV. Here, we assessed the sensitivity of ZZ-R 127 and LFBK-αVβ6 cells relative to primary BTY cells by titrating a range of FMDV original samples and isolates. Both the ZZ-R 127 and LFBK-αVβ6 cells were susceptible to FMDV for >100 passages, and there were no significant differences in sensitivity relative to primary BTY cells. Notably, the LFBK-αVβ6 cell line was highly sensitive to the O/CATHAY porcine-adapted FMDV strain. These results support the use of ZZ-R 127 and LFBK-αVβ6 as sensitive alternatives to BTY cells for the isolation of FMDV, and highlight the use of LFBK-αVβ6 cells as an additional tool for the isolation of porcinophilic viruses. |
Databáze: | OpenAIRE |
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