In vivo fluorescence imaging of conjunctival goblet cells
| Autor: | Moses Kim, Hoonchul Chang, Ki Hean Kim, Seunghun Lee, Seonghan Kim, Myoung Joon Kim |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Fluorescence-lifetime imaging microscopy Confocal Moxifloxacin lcsh:Medicine Article 03 medical and health sciences 0302 clinical medicine Microscopy medicine Fluorescence microscope Animals lcsh:Science Multidisciplinary Chemistry lcsh:R Optical Imaging Mucin Histology Translational research Fluorescence Rats Confocal microscopy 030104 developmental biology 030221 ophthalmology & optometry Specialized Epithelial Cell lcsh:Q Goblet Cells sense organs Conjunctiva |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-7 (2019) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-51893-4 |
| Popis: | Conjunctival goblet cells (GCs) are specialized epithelial cells that secrete mucins onto the ocular surface to maintain the wet environment. Assessment of GCs is important because various ocular surface diseases are associated with their loss. Although there are GC assessment methods available, the current methods are either invasive or difficult to use. In this report, we developed a simple and non-invasive GC assessment method based on fluorescence imaging. Moxifloxacin ophthalmic solution was used to label GCs via topical administration, and then various fluorescence microscopies could image GCs in high contrasts. Fluorescence imaging of GCs in the mouse conjunctiva was confirmed by both confocal reflection microscopy and histology with Periodic acid-Schiff (PAS) labeling. Real-time in-vivo conjunctival GC imaging was demonstrated in a rat model by using both confocal fluorescence microscopy and simple wide-field fluorescence microscopy. Different GC densities were observed in the forniceal and bulbar conjunctivas of the rat eye. Moxifloxacin based fluorescence imaging provides high-contrast images of conjunctival GCs non-invasively and could be useful for the study or diagnosis of GC related ocular surface diseases. |
| Databáze: | OpenAIRE |
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