Laminar Specificity of Local Circuits in Barrel Cortex of Ephrin-A5 Knockout Mice
| Autor: | Edward M. Callaway, Amy K. Butler, N. Harumi Yabuta |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Patch-Clamp Techniques
animal structures In Vitro Techniques Biology Mice medicine Animals Mice Knockout Cortical circuits Lysine Pyramidal Cells General Neuroscience Membrane Proteins Laminar flow Dendrites Somatosensory Cortex Barrel cortex Ephrin-A5 Axons medicine.anatomical_structure nervous system Knockout mouse Ephrin A5 Nerve Net Pyramidal cell Neuroscience Rapid Communication |
| Zdroj: | The Journal of Neuroscience. 20:RC88-RC88 |
| ISSN: | 1529-2401 0270-6474 |
| DOI: | 10.1523/jneurosci.20-15-j0005.2000 |
| Popis: | Cortical circuits are characterized by layer-specific axonal arbors. Molecular laminar cues are believed to direct the development of this specificity. We have tested the hypothesis that ephrin-A5 is responsible for preventing layer 2/3 pyramidal cell axons from branching within layer 4 (Castellani et al., 1998) by assessing the laminar specificity of axonal arbors in ephrin-A5 knockout mice. We find that in barrel cortex of knockout mice, layer 2/3 pyramidal neurons form axonal arbors specifically in layers 2/3 and 5, avoiding layer 4. This pattern of arborization is indistinguishable from that of wild-type littermates. Furthermore, we find that in wild-type mice, laminar patterns of ephrin-A5 expression differ between cortical areas despite the similarity of layer-specific local cortical circuits across areas. Most notably, ephrin-A5 is not expressed preferentially in layer 4 of wild-type mouse barrel cortex. We conclude that ephrin-A5 is not responsible for preventing the development of layer 2/3 pyramidal cell axonal arbors in layer 4 of mouse barrel cortex. These observations also suggest that if ephrin-A5 plays a role in the emergence of layer-specific circuits, that role must differ between cortical areas. |
| Databáze: | OpenAIRE |
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