Deployable, Field-Sustainable, Reverse Transcription-Polymerase Chain Reaction Assays for Rapid Screening and Serotype Identification of Dengue Virus in Mosquitoes
| Autor: | James C McAvin, Jamie A. Blow, William B. Huff, Michael D. Powers, John L. Putnam, James A. Swaby |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Time Factors Culex Pilot Projects Aedes aegypti Biology Dengue virus medicine.disease_cause Microbiology Dengue fever parasitic diseases medicine Animals Humans Mass Screening Serotyping Mass screening Aedes Reverse Transcriptase Polymerase Chain Reaction fungi Densovirinae Public Health Environmental and Occupational Health Original Articles General Medicine Dengue Virus Thailand biology.organism_classification medicine.disease Virology Culex tritaeniorhynchus Reverse transcription polymerase chain reaction Culicidae RNA Female Mobile Health Units |
| Zdroj: | Military Medicine |
| ISSN: | 1930-613X 0026-4075 |
| Popis: | Dengue virus universal and serotype 1 to 4 fluorogenic probe hydrolysis, reverse transcription (RT)-polymerase chain reaction (PCR) assays and positive-control RNA template were freeze-dried in a thermally stable, hydrolytic enzyme-resistant format and deployed for testing in a dengue fever-endemic region of Thailand. The study site presented austere testing conditions. Field-collected Aedes aegypti mosquitoes spiked with inoculated A. aegypti mosquitoes and individual and pooled, field-collected, A. aegypti, A. albopictus, and Culex tritaeniorhynchus mosquitoes were used for RT-PCR assay evaluations. For dengue virus-inoculated A. aegypti mosquitoes and spiked samples, in vitro sensitivity and specificity results for all five assays were concordant with indirect fluorescent antibody assay results. A single pool of field-collected, female, A. aegypti mosquitoes was identified as dengue virus positive. Cross-reactivity was not observed across heterologous serotypes, mosquito vectors, or human DNA. The limit of detection was >7 to ≤70 genomic equivalents. Sample processing and analysis required |
| Databáze: | OpenAIRE |
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