A Bioluminescent Biosensor for Quantifying the Interaction of SARS-CoV-2 and Its Receptor ACE2 in Cells and In Vitro
| Autor: | Xiaolong Yang, Prameet M. Sheth, Yu Tian Wang, Derek Zhang, Yanping Gong, Eva Y. So, Sahar Sarmasti Emami, Lidong Liu, Yawei Hao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
bioluminescent biosensor viruses Mutant ACE2 Biosensing Techniques In Vitro Techniques Microbiology Article RBD 03 medical and health sciences 0302 clinical medicine Protein Domains Virology Humans Bioluminescence Binding site Receptor Binding Sites Host Microbial Interactions SARS-CoV-2 Chemistry Drug discovery HEK 293 cells COVID-19 Antibodies Neutralizing QR1-502 In vitro High-Throughput Screening Assays NanoBiT Cell biology Luminescent Proteins HEK293 Cells 030104 developmental biology Infectious Diseases 030220 oncology & carcinogenesis Spike Glycoprotein Coronavirus therapeutic drugs Angiotensin-Converting Enzyme 2 Biosensor Protein Binding |
| Zdroj: | Viruses, Vol 13, Iss 1055, p 1055 (2021) Viruses Volume 13 Issue 6 |
| DOI: | 10.14288/1.0400152 |
| Popis: | The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is currently spreading and mutating with increasing speed worldwide. Therefore, there is an urgent need for a simple, sensitive, and high-throughput (HTP) assay to quantify virus–host interactions in order to quickly evaluate the infectious ability of mutant viruses and to develop or validate virus-inhibiting drugs. Here, we developed an ultrasensitive bioluminescent biosensor to evaluate virus–cell interactions by quantifying the interaction between the SARS-CoV-2 receptor binding domain (RBD) and its cellular receptor angiotensin-converting enzyme 2 (ACE2) both in living cells and in vitro. We have successfully used this novel biosensor to analyze SARS-CoV-2 RBD mutants and evaluated candidate small molecules (SMs), antibodies, and peptides that may block RBD:ACE2 interaction. This simple, rapid, and HTP biosensor tool will significantly expedite the detection of viral mutants and the anti-COVID-19 drug discovery process. |
| Databáze: | OpenAIRE |
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