Leishmania (Viannia) braziliensis: human mast cell line activation induced by logarithmic and stationary promastigote derived-lysates
| Autor: | Paulo R. Z. Antas, Claude Pirmez, Andrea S. Calheiros, Paula Melo De Luca, Marco A. Martins, Marcia Coronha R. Lima, Márcia Pereira de Oliveira |
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| Rok vydání: | 2005 |
| Předmět: |
Immunology
Enzyme-Linked Immunosorbent Assay Tryptase Histamine Release Leishmania braziliensis Cell Line Microbiology Mice chemistry.chemical_compound medicine Animals Humans Mast Cells biology Serine Endopeptidases General Medicine Lipophosphoglycan Flow Cytometry Leishmania biology.organism_classification Mast cell Immunohistochemistry Interleukin-12 Molecular biology Infectious Diseases medicine.anatomical_structure chemistry Cell culture Interleukin 12 biology.protein Cytokines Tryptases Parasitology Interleukin-4 Histamine |
| Zdroj: | Experimental Parasitology. 109:72-79 |
| ISSN: | 0014-4894 |
| DOI: | 10.1016/j.exppara.2004.11.011 |
| Popis: | Herein we investigate the ability of live promastigotes and total lysate of Leishmania (Viannia) braziliensis, derived from parasites in the logarithmic (L-Lb) or stationary phase (S-Lb), to induce human mast cell line (HMC-1) activation. In comparison with medium-treated cells, a significant histamine release was observed in HMC-1 cultures stimulated with S-Lb. Lipophosphoglycan also induced histamine release by HMC-1 cells. In immunocytochemical assays, we found a marked staining for tryptase in medium-treated HMC-1 cells, however, stimulation with L-Lb or S-Lb caused a marked decrease in the color reaction as well as in the number of tryptase-positive cells. L-Lb and S-Lb induced an evident decrease in the intracellular expression of IL-4 but not IL-12. Live stationary promastigotes were able to induce high levels of IL-4 release in HMC-1 cultures. Furthermore, these cells released significant amounts of IL-12 when incubated with both types of live promastigotes. These results indicate that L. (V.) braziliensis promastigotes differ in their ability to induce direct human mast cells activation, according to the growth phase of the parasite. Furthermore, the release of pro-inflammatory mediators and cytokines could represent an important phenomenon that might favor the initial establishment of the infection. |
| Databáze: | OpenAIRE |
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