Serum-free primary culture of human normal mammary epithelial cells in collagen gel matrix
| Autor: | Darragh Flynn, Lisa Larson, S. Nandi, Joel J. Elias, Jason Yang |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
medicine.medical_specialty
Cholera Toxin Primary culture Hydrocortisone medicine.medical_treatment Biology Matrix (biology) medicine.disease_cause Epidermal growth factor Internal medicine medicine Methods Humans Insulin Breast Cells Cultured chemistry.chemical_classification Epidermal Growth Factor Cholera toxin Transferrin Epithelial Cells Serum Albumin Bovine Cell Biology Molecular biology Collagen gel Culture Media Endocrinology chemistry Female Collagen Gels Hormone |
| Zdroj: | Cell biology international reports. 6(10) |
| ISSN: | 0309-1651 |
| Popis: | Collagen gel matrix has been used successfully to promote sustained growth of human normal mammary epithelial cells in primary culture using serum-containing medium supplemented with hormones and growth factors (Nandi et al., 1932). Sustained growth can now be accomplished in a serum-free medium consisting of a 1:1 mixture of Ham's F12 and DMEM supplemented with insulin, transferrin, epidermal growth factor, cholera toxin, cortisol, and BSA. Human normal mammary epithelial cells derived from reduction mammoplasties can be routinely propagated in this serum-free medium. The extent of growth and the resulting three-dimensional outgrowths in this serum-free medium, using the collagen gel matrix system, are comparable to those seen in serum-containing medium. This is the first demonstration of sustained growth of human normal mammary epithelial cells in serum-free primary culture. |
| Databáze: | OpenAIRE |
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