The Ribosomal Shunt Translation Strategy of Cauliflower Mosaic Virus Has Evolved from Ancient Long Terminal Repeats
| Autor: | June E. Bourque, Dong Xu, Monir Shababi, James E. Schoelz, Karuppaiah Palanichelvam, Xiu-Feng Wan, Anthony B. Cole |
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| Rok vydání: | 2006 |
| Předmět: |
Agroinfiltration
Retroelements Molecular Sequence Data Immunology Saccharomyces cerevisiae Retrotransposon Biology Microbiology Genome Open Reading Frames Caulimovirus Virology Promoter Regions Genetic Genetics Base Sequence fungi Terminal Repeat Sequences food and beverages Ribosomal RNA biology.organism_classification Biological Evolution Long terminal repeat Genetic translation Plant Leaves Enhancer Elements Genetic Genetic Diversity and Evolution Protein Biosynthesis Insect Science Cauliflower mosaic virus Ribosomes |
| Zdroj: | Journal of Virology. 80:3811-3822 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.80.8.3811-3822.2006 |
| Popis: | We have screened portions of the large intergenic region of the Cauliflower mosaic virus (CaMV) genome for promoter activity in baker's yeast ( Saccharomyces cerevisiae ) and have identified an element that contributes to promoter activity in yeast but has negligible activity in plant cells when expressed in an agroinfiltration assay. A search of the yeast genome sequence revealed that the CaMV element had sequence similarity with the R region of the long terminal repeat (LTR) of the yeast Ty1 retrotransposon, with significant statistical confidence. In plants, the same CaMV sequence has been shown to have an essential role in the ribosomal shunt mechanism of translation, as it forms the base of the right arm of the stem-loop structure that is required for the ribosomal shunt. Since the left arm of the stem-loop structure must represent an imperfect reverse copy of the right arm, we propose that the ribosomal shunt has evolved from a pair of LTRs that have become incorporated end to end into the CaMV genome. |
| Databáze: | OpenAIRE |
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