Structure of Urocanate Hydratase from the protozoan Trypanosoma cruzi
| Autor: | Sheila Boreiko, Marcio Silva, Ariel Mariano Silber, Raíssa de F. P. Melo, Jorge Iulek |
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| Rok vydání: | 2020 |
| Předmět: |
Protein Conformation
alpha-Helical Trypanosoma cruzi 02 engineering and technology Bacillus subtilis MUTAÇÃO Biochemistry 03 medical and health sciences chemistry.chemical_compound Structural Biology Urocanate Hydratase Histidine Amino Acid Sequence Cloning Molecular Molecular Biology 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology Pseudomonas putida Chemistry Geobacillus Reproducibility of Results Enzyme Interaction General Medicine NAD 021001 nanoscience & nanotechnology biology.organism_classification Monomer Enzyme NAD+ kinase Crystallization 0210 nano-technology Sequence Alignment |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2019.12.101 |
| Popis: | The enzyme Urocanate Hydratase (UH) participates in the catabolic pathway of L-histidine. Trypanosoma cruzi Urocanate Hydratase (TcUH) is identified as a therapeutic molecular target in the WHO/TDR Targets Database. We report the 3D structure determination and number of features of TcUH, and compared it to other few available bacterial UH structures. Each monomer presents two domains and one NAD+ molecule. Superpositions revealed differences in the relative orientation of domains within monomers, such that TcUH monomer A resembles Urocanate Hydratase from Geobacillus kaustophilus (GkUH) (open conformation), while monomer C resembles Urocanate Hydratase from Pseudomonas putida (PpUH) and Urocanate Hydratase from Bacillus subtilis (BsUH) (closed conformations). We use the structure of TcUH to make considerations about 3 non-deleterious and 2 deleterious mutations found in human UHs: non-deleterious mutations could be accommodated without large displacements or interaction interruptions, whereas deleterious mutations in one case might disrupt an α-helix (as previously suggested) and in the other case, besides disrupting the enzyme interaction with the substrate, might interfere with interdomain movement. |
| Databáze: | OpenAIRE |
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