Detection and modeling of anti-Leptospira IgG prevalence in cats from Lisbon area and its correlation to retroviral infections, lifestyle, clinical and hematologic changes
| Autor: | Luís Tavares, Rodolfo Oliveira Leal, Joana Moreira da Silva, Telmo Nunes, Solange Gil, Sara Prata, Nuno Sepúlveda, Virgílio Almeida, Tiago Dias Domingues |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
CBC
complete blood count 040301 veterinary sciences IgG animal diseases AST aspartate aminotransferase Article IDIU Infectious Diseases Isolation Unit 0403 veterinary science Antigen Leptospira ALT alanine aminotransferase Free-roaming cats medicine MAT microscopic agglutination tests Seroprevalence Leptospirosis One Health VTH Veterinary Teaching Hospital WHO World Health organization CATS lcsh:Veterinary medicine IgG Immunoglobulin G General Veterinary biology business.industry PAHO Pan American Health organization Zoonosis FIV positive 0402 animal and dairy science ELISA enzyme-linked immunosorbent assay 04 agricultural and veterinary sciences bacterial infections and mycoses medicine.disease biology.organism_classification 040201 dairy & animal science USG Urine Specific Gravity ALP serum alkaline phosphatase Immunology biology.protein bacteria lcsh:SF600-1100 Animal Science and Zoology Antibody FIV Feline Immunodeficiency Virus infection business Asymptomatic carrier CKD Chronic Kidney Disease FeLV Feline Leukemia Virus |
| Zdroj: | Veterinary and Animal Science, Vol 10, Iss, Pp 100144-(2020) Veterinary and Animal Science |
| Popis: | Highlights • Anti-Leptospira IgG seroprevalence was estimated on cats from Lisbon, Portugal via ELISA. • A mathematical model was applied to raw data to establish the real cut-off value of seroprevalence. • Of the 243 samples, 59.3% tested positive for anti-Leptospira IgG. • A positive correlation between low anti-Leptospira IgG and FIV+ was detected (p = 0.02). • No correlation was detected between anti-Leptospira IgG values and outdoor lifestyle. Leptospirosis is a zoonosis of global importance caused by Leptospira species. Rodents are the main reservoirs, known to shed the bacteria in urine, thus contaminating water and soil and infecting other animals and people. Leptospirosis has been re-emerging in both developing and developed countries including Europe. It has been hypothesized that cats could be asymptomatic carriers of Leptospira. This study aims to evaluate cats’ exposure to Leptospira in Lisbon, Portugal, by measuring IgG titres and correlating them with possible factors that may increase the risk of exposure in urban cats. Two hundred and forty-three samples were collected from the biobank. An ELISA test followed by a seroprevalence analysis using a finite mixture model was performed to detect and measure anti-Leptospira IgG antibodies titres. In parallel, a survey was conducted to identify possible risk factors for seropositivity. According to the ELISA test protocol, only twenty-three cats (9.5%; 95% CI =(6.1%;13.9%)) could be considered as seropositive to Leptospira antigens. However, when the same data were analysed by the best different mixture models, one hundred and forty-four cats (59.3%; 95%CI = (52.8%; 65.5%)) could be classified as intermediate and high antibody responders to Leptospira antigens. Seropositivity to Feline Immunodeficiency Virus infection (FIV) was found to be the only significant risk factor associated with anti-Leptospira IgG antibodies. In conclusion, the present studies raises the possibility of a higher exposure of cats to Leptospira than previously thought due to the identification of a subpopulation of cats with intermediate antibody levels. |
| Databáze: | OpenAIRE |
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