Muscarinic receptor subtypes modulate the release of [3H]-noradrenaline in rat spinal cord slices
| Autor: | Yoshihiko Aramaki, Tomohisa Mori, Tomiei Kazama, Eiichiro Umeda |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
medicine.medical_specialty Muscarinic Antagonists In Vitro Techniques Tritium Rats Sprague-Dawley Norepinephrine Internal medicine Muscarinic acetylcholine receptor medicine Muscarinic acetylcholine receptor M4 Animals Drug Interactions Dose-Response Relationship Drug Chemistry General Neuroscience Muscarinic acetylcholine receptor M3 Muscarinic acetylcholine receptor M2 Muscarinic acetylcholine receptor M1 Receptors Muscarinic Pirenzepine Neostigmine Rats Endocrinology Spinal Cord Cholinesterase Inhibitors Acetylcholine medicine.drug |
| Zdroj: | Brain Research Bulletin. 70:99-102 |
| ISSN: | 0361-9230 |
| DOI: | 10.1016/j.brainresbull.2006.04.002 |
| Popis: | Spinal muscarinic receptors are involved in the mediation of antinociceptive effects. The modulation of noradrenaline (NA) release on muscarinic receptor subtypes in the rat spinal cord was investigated in in vitro perfusion experiments. After rat spinal cord slices were preincubated in [3H]NA, the slices were perfused with a superfusion apparatus. The slices were field stimulated during the 4th (S1) and 11th (S2) superfusion collection periods. Perfusion of drugs was initiated at the 8th collection period and was maintained until the 14th collection period. Fractional release was calculated as the percentage of the radioactivity present in the slices at the beginning of the stimulation period. Drugs were administered between S1 and S2. The following drugs were used: [3H]NA, neostigmine, pirenzepine (M1 antagonist), AFDX116 (M2 antagonist), atropine. Neostigmine significantly increased the release of [3H]NA in a concentration-dependent manner. Pirenzepine (1 microM) and atropine (0.3 microM) significantly reduced the release of [3H]NA, but AFDX116 (1 microM) did not significantly reduce release in the presence of neostigmine (1 microM). The results of this study indicate that neostigmine can enhance noradrenergic neurotransmission, and that acetylcholine can stimulate spinal cord NA release via M1 muscarinic receptor subtypes. |
| Databáze: | OpenAIRE |
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