Comparison of minimal residual disease detection in multiple myeloma by SRL 8-color single-tube and EuroFlow 8-color 2-tube multiparameter flow cytometry
| Autor: | Kazuya Kobori, Kosei Matsue, Masako Hanawa, Takeshi Yoroidaka, Momoko Fujisawa, Takeshi Yamashita, Mikio Ueda, Hiroyuki Takamatsu, Ryoichi Murata, Shinji Nakao |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male medicine.medical_specialty Neoplasm Residual medicine.drug_class Bone Marrow Cells Monoclonal antibody 03 medical and health sciences 0302 clinical medicine Antigens CD EuroFlow hemic and lymphatic diseases Internal medicine medicine Humans Multiparameter flow cytometry Multiple myeloma Aged Aged 80 and over Hematology biology business.industry Middle Aged Flow Cytometry medicine.disease Minimal residual disease Neoplasm Proteins medicine.anatomical_structure 030220 oncology & carcinogenesis biology.protein Female Bone marrow Antibody Multiple Myeloma business Nuclear medicine 030215 immunology |
| Zdroj: | International Journal of Hematology. 109:377-381 |
| ISSN: | 1865-3774 0925-5710 |
| Popis: | We sought to determine the efficacy of a new, inexpensive, single-tube 8-color multiparameter flow cytometry (MFC) method (SRL-Flow), which is based on the EuroFlow next-generation flow (NGF) (tube 2 only), to assess minimal residual disease (MRD)-negative status. MRD-negative status is considered a treatment milestone in multiple myeloma (MM). We used 45 bone marrow samples from patients with MM, including 11 cases treated with anti-CD38 monoclonal antibody. The SRL-Flow sample preparation protocol was identical to that of EuroFlow-NGF. The antibody panel for SRL-Flow was as follows: CD138V450/CD27V500/CD38ME (multiepitope)FITC/CD56PE/CD45PerCP-Cy5.5/CD19PE-Cy7/cytoplasmic (Cy) immunoglobulin (Ig) κAPC/CyIgλAPC-H7. To identify abnormal plasma cells (aPCs) of patients with MM who received anti-CD38 monoclonal antibody, we used a panel of anti-CD45 and anti-CD138 antibodies (Abs) rather than a panel of anti-CD45 and anti-CD38 Abs. We comparatively analyzed the total nucleated cell numbers, total PC levels, and MRD levels between the SRL-Flow and EuroFlow-NGF. High correlations (r > 0.9) in total PC and MRD levels were noted among SRL-Flow, original EuroFlow-NGF (2 tubes), and EuroFlow-NGF (tube 2 only), suggesting that SRL-Flow is an inexpensive (< $200 USD/sample as of January of 2019) alternative to EuroFlow-NGF (< $350 USD/sample) for assessing MRD in MM. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink