Synchrotron radiation microbeam X-ray fluorescence analysis of zinc concentration in remineralized enamel in situ
| Autor: | Yoshihiko Hayashi, Hidetaka Ishizaki, Tsunenori Matsunaga, Shuji Tanabe |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Calcium Phosphates
Silicon Materials science Analytical chemistry X-ray fluorescence Mineralogy chemistry.chemical_element Zinc Dental Caries Lithium Chewing Gum Double-Blind Method stomatognathic system Polysaccharides Humans Dental Enamel General Dentistry Xylitol Remineralisation Crystallography Extended X-ray absorption fine structure Enamel paint Synchrotron radiation Spectrometry X-Ray Emission Cell Biology General Medicine Microbeam Tooth Remineralization Oxygen stomatognathic diseases Durapatite Otorhinolaryngology chemistry visual_art visual_art.visual_art_medium Synchrotrons Remineralization Electron Probe Microanalysis |
| Zdroj: | Archives of oral biology. 54(5):420-423 |
| ISSN: | 0003-9969 |
| Popis: | OBJECTIVE: Remineralization is an indispensable phenomenon during the natural healing process of enamel decay. The incorporation of zinc (Zn) into enamel crystal could accelerate this remineralization. The present study was designed to investigate the concentration and distribution of Zn in remineralized enamel after gum chewing. METHODS: The experiment was performed at the Photon Factory. Synchrotron radiation was monochromatized and X-rays were focused into a small beam spot. The X-ray fluorescence (XRF) from the sample was detected with a silicon (Si) (lithium (Li)) detector. X-ray beam energy was tuned to detect Zn. The examined samples were small enamel fragments remineralized after chewing calcium phosphate-containing gum in situ. The incorporation of Zn atom into hydroxyapatite (OHAP), the main component of enamel, was measured using Zn K-edge extended X-ray absorption fine structure (EXAFS) with fluorescence mode at the SPring-8. RESULTS: A high concentration of Zn was detected in a superficial area 10-mum deep of the sectioned enamel after gum chewing. This concentration increased over that in the intact enamel. The atomic distance between Zn and O in the enamel was calculated using the EXAFS data. The analyzed atomic distances between Zn and O in two sections were 0.237 and 0.240nm. CONCLUSION: The present experiments suggest that Zn is effectively incorporated into remineralized enamel through the physiological processes of mineral deposition in the oral cavity through gum-chewing and that Zn substitution probably occurred at the calcium position in enamel hydroxyapatite. Archives of Oral Biology, 54(5), pp.420-423; 2009 |
| Databáze: | OpenAIRE |
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