Prevalence and molecular characterization of porcine Picobirnavirus in piglets of North East Region of India
Autor: | Y. S. Malik, Tapan Kumar Dutta, P. K. Subudhi, Parimal Roychoudhury, Rajkumari Mandakini, Hosterson Kylla |
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Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Diarrhea Veterinary medicine Swine 030106 microbiology Prevalence India Picobirnavirus Crossbreed 03 medical and health sciences Feces Animal science RNA Virus Infections Food Animals medicine Animals Cloning Molecular Phylogeny RNA Double-Stranded Porcine picobirnavirus Swine Diseases Molecular Epidemiology Molecular epidemiology biology Sewage biology.organism_classification Breed Gastroenteritis 030104 developmental biology Piglets Animal Science and Zoology Seasons medicine.symptom Water Microbiology Regular Articles |
Zdroj: | Tropical Animal Health and Production |
ISSN: | 1573-7438 0049-4747 |
Popis: | Picobirnaviruses (PBVs) have been recognized as one of the important causal viral agents of gastroenteritis in several animal species especially in young immunocompromised hosts. In this study, we report the prevalence and molecular epidemiology of porcine PBVs from North Eastern Hilly region of India. A total of 457 fecal samples from piglets were collected from local (n = 130) and cross (n = 327) breed piglets in different seasons for 2 years. All the samples were subjected to RNA-PAGE and RT-PCR analysis for detection of PBVs. A total of 4.59 and 11.15% samples were recorded as positive for PBVs by RNA-PAGE and RT-PCR, respectively. Rate of detection was higher from diarrhoeic animals (13.56%) compared to non-diarrhoeic (4.23%) animals. Higher prevalence rate was observed from unorganized farms (14.22%) compared to organized farms (8.0%) with slightly higher detection from cross breed (11.62%) compared to local breed (10.0%). Maximum cases of piglet diarrhea associated with PBVs were detected during summer (16.4%) and winter (14.39%) seasons compared to autumn (4.80%) and spring (6.45%). All the samples were positive for PBV genogroup I only. Based upon the sequence analysis, the isolates were unique and placed in separate clad and were not closely associated with any other Indian isolates of PBVs so far. Two isolates were closely related with one Chinese isolate recovered from sewage water. This is the first systematic study of prevalence of PBVs associated with piglet diarrhea. |
Databáze: | OpenAIRE |
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