The bifactorial endosperm box of gamma-zein gene: characterisation and function of the Pb3 and GZM cis-acting elements
| Autor: | M D Ludevid, Margarita Torrent, P K Busk, Pau Marzábal |
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| Přispěvatelé: | Ministerio de Educación y Ciencia (España), Danish Natural Science Research Council |
| Rok vydání: | 1998 |
| Předmět: |
Saccharomyces cerevisiae Proteins
DNA Plant Zein Molecular Sequence Data DNA Footprinting DNA footprinting Plant Science Biology Fungal Proteins Glutelin Transcription (biology) Sequence Homology Nucleic Acid Gene expression Genetics Promoter Regions Genetic Gene Transcription factor Plant Proteins Base Sequence Nucleic acid sequence Cell Biology Molecular biology DNA-Binding Proteins Molecular Weight Enhancer Elements Genetic Regulatory sequence biology.protein Protein Kinases Prolamins |
| Zdroj: | Europe PubMed Central Roskilde University Digital.CSIC. Repositorio Institucional del CSIC instname Peter Kamp Busk |
| ISSN: | 0960-7412 |
| Popis: | The proximal region of the γ-zein promoter (γ Z ) has a functional bifactorial prolamin box element containing two cis -acting elements, a prolamin-box motif (Pb3) and a GCN4-like motif (GZM). By particle bombardment of maize endosperms with 5′ deletions and internal deletions of γ Z fused to the GUS gene, we have shown that a 135 bp region containing the bifactorial element is involved in the transcriptional activation of the γ Z promoter. However, the 135 bp region was unable to activate the γ Z promoter in the absence of a 84 bp downstream sequence. Using in vivo footprinting and gel mobility shift assays with 15 DAP endosperm nuclear extracts, we have demonstrated the presence of trans -acting factors that interact with Pb3 and GZM target sites. Base-substitution mutations within Pb3 and GZM decreased transcription activity of the γ Z promoter suggesting a co-ordinated function between the two cis -acting elements. Two additional cis -motifs upstream of the bifactorial prolamin element have been identified: a motif with high homology to the AACA elements of rice glutelin genes and an AZM motif containing an ACGT core which binds nuclear proteins other than the Opaque 2 (O2). This work was supported by Plan Nacional de Biotecnologia (Bio95–070). P.M. is a fellow of Ministerio de Educación y Ciencia and P.K.B. was supported by a fellowship from the Danish Natural Science Research Council. |
| Databáze: | OpenAIRE |
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