Cortactin is required for integrin-mediated cell spreading
| Autor: | Gábor Bogel, Annamária Balázs, Péter Tamás, Balázs Enyedi, László Buday, András Illés, Lukács, Melinda |
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| Rok vydání: | 2006 |
| Předmět: |
Transcriptional Activation
Integrins Recombinant Fusion Proteins Green Fluorescent Proteins Immunology Integrin macromolecular substances SH3 domain Cell Movement RNA interference Chlorocebus aethiops Cell Adhesion Animals Point Mutation Immunology and Allergy RNA Small Interfering Cell adhesion COS cells biology Chemistry Fibronectins Protein Structure Tertiary Cell biology Fibronectin COS Cells biology.protein RNA Interference Lamellipodium Cortactin |
| Zdroj: | Immunology Letters. 104:124-130 |
| ISSN: | 0165-2478 |
| Popis: | Cortactin is an SH3 domain-containing protein that contributes to the formation of dynamic cortical actin-associated structures, such as lamellipodia and membrane ruffles. Here we show that expression of either the GFP-tagged N-terminal or the C-teminal halves of cortactin inhibits significantly the spreading of COS7 cells on fibronectin. Introducing inactivating point mutation into the SH3 domain of the C-terminal half of cortactin suspends the dominant negative effect of the construct. In addition, a vector-based RNA interference was used to knock-down endogenous level of cortactin in cells. We demonstrate that cortactin deficient cells were not able to spread. These results suggest that cortactin is required for integrin-mediated signalling pathways. |
| Databáze: | OpenAIRE |
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