Circ_0020093 ameliorates IL-1β-induced apoptosis and extracellular matrix degradation of human chondrocytes by upregulating SPRY1 via targeting miR-23b
Autor: | Jiang Huang, Shuai An, Jingbo Cheng, Mingli Feng, Qi Yan, Lin Jing |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
SPRY1 Clinical Biochemistry Interleukin-1beta Apoptosis Chondrocyte Article Extracellular matrix 03 medical and health sciences 0302 clinical medicine Chondrocytes Western blot circ_0020093 Osteoarthritis medicine Humans Molecular Biology miR-23b Reporter gene Gene knockdown Messenger RNA medicine.diagnostic_test Chemistry Membrane Proteins Cell Biology General Medicine RNA Circular Phosphoproteins Cell biology Extracellular Matrix Up-Regulation MicroRNAs 030104 developmental biology medicine.anatomical_structure IL-1β 030220 oncology & carcinogenesis Extracellular Matrix Degradation |
Zdroj: | Molecular and Cellular Biochemistry |
ISSN: | 1573-4919 0300-8177 |
Popis: | Osteoarthritis (OA) is a chronic disease characterized by articular cartilage degeneration and uncontrolled chondrocyte apoptosis. At present, accumulating evidence introduces that circular RNA (circRNA) is involved in the development of OA. The aim of our study was to explore the role and the functional mechanism of circ_0020093 in OA cell model. Human chondrocytes were treated with interleukin-1 beta (IL-1β) to construct OA model. The expression of circ_0020093, miR-23b, and Sprouty 1 (SPRY1) mRNA was measured by quantitative real-time polymerase chain reaction (qRT-PCR). Cell apoptosis was assessed by flow cytometry assay. The expression of extracellular matrix (ECM)-associated markers and SPRY1 protein level was detected by qRT-PCR and Western blot. Bioinformatics analysis-predicted relationship between miR-23b and circ_0020093 or SPRY1 was further verified by dual-luciferase reporter assay and RNA pull-down assay. In this study, we found that the expression of circ_0020093 and SPRY1 was declined, while miR-23b expression was elevated in IL-1β-treated chondrocytes. IL-1β induced chondrocyte apoptosis and ECM degradation, while these negative effects were alleviated by circ_0020093 overexpression or miR-23b inhibition. MiR-23b was a target of circ_0020093, and SPRY1 was a downstream target of miR-23b. Rescue experiments showed that miR-23b enrichment reversed the role of circ_0020093 overexpression, and SPRY1 knockdown also reversed the effects of miR-23b inhibition. Importantly, circ_0020093 positively regulated SPRY1 expression by targeting miR-23b. In conclusion, circ_0020093 ameliorates IL-1β-induced apoptosis and ECM degradation of human chondrocytes by regulating the miR-23b/SPRY1 axis. Supplementary Information The online version contains supplementary material available at 10.1007/s11010-021-04186-2. |
Databáze: | OpenAIRE |
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