Sulforaphane Modulates AQP8-Linked Redox Signalling in Leukemia Cells
| Autor: | Cecilia Prata, Carlotta Facchini, Emanuela Leoncini, Monia Lenzi, Tullia Maraldi, Cristina Angeloni, Laura Zambonin, Silvana Hrelia, Diana Fiorentini, ANGELONI, CRISTINA |
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| Přispěvatelé: | Cecilia Prata , Carlotta Facchini, Emanuela Leoncini, Monia Lenzi , Tullia Maraldi , Cristina Angeloni , Laura Zambonin, Silvana Hrelia , Diana Fiorentini |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Aging Cell signaling Article Subject Cell Survival Cell LEUKEMIA CELLS Aquaporins Biochemistry Cell Line Cell Line Tumor Cell Membrane Cell Proliferation Humans Hydrogen Peroxide Isothiocyanates Leukemia NADPH Oxidase 2 Peroxiredoxins Signal Transduction Cell Biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine REACTIVE OXYGEN SPECIES lcsh:QH573-671 Tumor lcsh:Cytology Cell growth Myeloid leukemia SULFORAPHANE General Medicine medicine.disease Cell biology 030104 developmental biology medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Sulfoxides Cancer cell Signal transduction MEMBRANE Sulforaphane Research Article |
| Zdroj: | Oxidative Medicine and Cellular Longevity Oxidative Medicine and Cellular Longevity, Vol 2018 (2018) |
| ISSN: | 1942-0994 |
| Popis: | Sulforaphane, a biologically active isothiocyanate compound extracted from cruciferous vegetables, has been shown to exert cytotoxic effects on many human cancer cells, including leukemia. However, the exact molecular mechanisms behind the action of sulforaphane in hematological malignancies are still unclear. Like other cancer cells, leukemia cells produce high level of reactive oxygen species; in particular, hydrogen peroxide derived from Nox family is involved in various redox signal transduction pathways, promoting cell proliferation and survival. Recent evidence show that many tumour cell types express elevated level of aquaporin isoforms, and we previously demonstrated that aquaporin-8 acts as H2O2 transport facilitator across the plasma membrane of B1647 cells, a model of acute myeloid human leukemia. Thus, the control of AQP8-mediated H2O2 transport could be a novel strategy to regulate cell signalling and survival. To this purpose, we evaluated whether sulforaphane could somehow affect aquaporin-8-mediated H2O2 transport and/or Nox-mediated H2O2 production in B1647 cell line. Results indicated that sulforaphane inhibited both aquaporin-8 and Nox2 expression, thus decreasing B1647 cells viability. Moreover, the data obtained by coimmunoprecipitation technique demonstrated that these two proteins are linked to each other; thus, sulforaphane has an important role in modulating the downstream events triggered by the axis Nox2-aquaporin-8. Cell treatment with sulforaphane also reduced the expression of peroxiredoxin-1, which is increased in almost all acute myeloid leukemia subtypes. Interestingly, sulforaphane concentrations able to trigger these effects are achievable by dietary intake of cruciferous vegetables, confirming the importance of the beneficial effect of a diet rich in bioactive compounds. |
| Databáze: | OpenAIRE |
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