Patterns of matrix metalloproteinase and TIMP-1 expression in chronic and normally healing human cutaneous wounds
| Autor: | J. Lauharanta, Juha Kere, Ulpu Saarialho-Kere, Maarit Vaalamo, Pia Saarinen, Pauli Puolakkainen, M Weckroth |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Chronic wound
Pathology medicine.medical_specialty 0303 health sciences integumentary system Matrix metalloproteinase inhibitor Surgical wound Inflammation In situ hybridization Dermatology Biology Matrix metalloproteinase 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine 030220 oncology & carcinogenesis medicine Collagenase Matrilysin medicine.symptom medicine.drug 030304 developmental biology |
| Zdroj: | British Journal of Dermatology. 135:52-59 |
| ISSN: | 1365-2133 0007-0963 |
| DOI: | 10.1111/j.1365-2133.1996.tb03607.x |
| Popis: | The present study was carried out to characterize the patterns of expression of matrix metalloproteinases or their tissue inhibitor (TIMP-1) in normally healing, acute vs. chronic, skin wounds. In situ hybridization was performed to localize collagenase, stromelysin-1, stromelysin-2, matrilysin, urokinase plasminogen activator (uPA) and TIMP-1 mRNAs in 14 chronic venous ulcers and 10 normally healing wounds, representing different time points after wounding. Surgical wounds, made in piglets harvested at several time points, were studied as controls. Collagenase, stromelysin-1 and -2, as well as uPa, were expressed in keratinocytes in both acute and chronic wounds, while epithelial TIMP-1 mRNA was not detected in any chronic wound biopsies studied. However, TIMP-1 was expressed at the epithelial edges of both acute human and pig wounds. Our results suggest that the balance between metalloenzymes and their inhibitor TIMP-1, is disturbed, in poorly healing wounds. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text