A minimally invasive, low-stress method for serial blood collection in aging mice
| Autor: | Lida Zhu, Marianne Bjorner |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Aging
medicine.medical_specialty Pathology Histology 040301 veterinary sciences Blood volume lcsh:Geriatrics Cell morphology 0403 veterinary science 03 medical and health sciences Immune system Technical Report White blood cell Internal medicine medicine 030304 developmental biology 0303 health sciences Hematology business.industry hematology 04 agricultural and veterinary sciences Blood collection Staining lcsh:RC952-954.6 aging mice white blood cell differentiation medicine.anatomical_structure Geriatrics and Gerontology Blood collection protocol business Blood sampling |
| Zdroj: | Pathobiology of Aging & Age Related Diseases Pathobiology of Aging & Age-related Diseases, Vol 9, Iss 1 (2019) |
| ISSN: | 2001-0001 |
| Popis: | Hematologic analysis is an efficient and valuable tool for real-time health monitoring and immune analysis in mouse aging studies. However, many frequently used blood sampling techniques in mice are incompatible with continuous monitoring with increasing age, as they may involve anesthesia, cause severe stress, or require a high volume of blood. This technical report describes a convenient relatively noninvasive procedure for counting white blood cells in C57Bl/6 mice by an optimized tail blood collection method followed by Wright-Giemsa and Türk staining. This technique can be performed on unanesthetized mice in less than 1 min with minimal stress using only a few microliters of blood. White blood cell analysis can include but is not limited to total and differential white blood cell count and cell morphology. The low blood volume collected is ideal for aging mice in longer-term experiments requiring regular and continuous monitoring. |
| Databáze: | OpenAIRE |
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