Effect of 6-Azacytidine on the Course of Experimental Adenoviral Infection in Newborn Syrian Hamsters
| Autor: | A.V. Slita, N. S. Dyachenko, V. L. Zhovnovataya, L. N. Nosach, V.P. Sukhinin, L. I. Palchikovskaya, Olga Povnitsa, V.V. Zarubaev, I. V. Alexeeva |
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| Rok vydání: | 2007 |
| Předmět: |
Adenoviridae Infections
Pharmacology medicine.disease_cause Antiviral Agents Virus In vivo Cricetinae medicine Animals Pharmacology (medical) Respiratory system Kidney Mesocricetus biology Adenoviruses Human biology.organism_classification Mastadenovirus Adenoviridae Disease Models Animal Infectious Diseases medicine.anatomical_structure Animals Newborn Oncology Immunology Toxicity Azacitidine Spleen |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1973-9478 1120-009X |
| DOI: | 10.1179/joc.2007.19.1.44 |
| Popis: | Adenoviral infection is a serious human pathology leading to respiratory, gastrointestinal and ocular disorders and epidemic outbreaks, especially in children's groups. Here we present the results from an investigation of anti- adenoviral effect of 6-azacytidine (6-AC) both in vitro and in vivo. The selectivity index of 6-AC for adenovirus type 5 in HEp-2 cells was 374, the 50% effective concentration was 0.5 mg/ml. For in vivo investigations we developed a model of disseminated adenoviral infection in newborn Syrian hamsters. The infectious virus was recovered from the liver, kidney, lungs and heart. Application of 6-AC led to a reduced period of the virus presence (7 days in the liver and 4 days in the kidney and heart) and lowered virus titers on day 3 post-inoculation (p.i.) (liver - 2.7 and 4.1, heart - 0 and 3.2, kidney - 0 and 2.4 log(10 )CPD(50)/mg tissue weight, in the presence and absence of 6-AC, respectively). Application of 6-AC to newborn Syrian hamsters led to partial destruction of their splenocytes. The results obtained suggest that 6-AC or 6-ACbased drugs with lower toxicity or applied topically may be suitable for therapy and prevention of adenoviral infection in humans. |
| Databáze: | OpenAIRE |
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