Proline oxidation fuels mitochondrial respiration during dark-induced leaf senescence in Arabidopsis thaliana
| Autor: | Pierre Carol, Séverine Planchais, Arnould Savouré, Cécile Cabassa-Hourton, Luc Richard, Holger Eubel, Régis Maldiney, Gilles Clément, Marianne Bordenave-Jacquemin, Alban Launay, Jérôme Lacoste, Hans-Peter Braun, Anne Guivarc'h, Sandrine Lebreton, Emilie Crilat |
|---|---|
| Přispěvatelé: | Institut d'écologie et des sciences de l'environnement de Paris (iEES), Centre National de la Recherche Scientifique (CNRS)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Recherche Agronomique (INRA), Leibniz University Hannover, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Sorbonne University, Institut d'écologie et des sciences de l'environnement de Paris (IEES), Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Senescence Chlorophyll Proline Arabidopsis thaliana Physiology Cell Respiration Arabidopsis Plant Science Mitochondrion 01 natural sciences 03 medical and health sciences Proline dehydrogenase Oxygen Consumption Gene Expression Regulation Plant proline metabolism Amino Acid Sequence [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] Dewey Decimal Classification::500 | Naturwissenschaften::560 | Paläontologie 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology Catabolism Arabidopsis Proteins dark-induced leaf senescence primary metabolism Darkness [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics biology.organism_classification Research Papers Amino acid [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biomolecules [q-bio.BM] Citric acid cycle Plant Leaves mitochondria Biochemistry chemistry ddc:560 Metabolome Growth and Development proline dehydrogenase Oxidation-Reduction 010606 plant biology & botany |
| Zdroj: | Journal of Experimental Botany 70 (2019), Nr. 21 Journal of Experimental Botany Journal of Experimental Botany, Oxford University Press (OUP), 2019, 70 (21), pp.6203-6214. ⟨10.1093/jxb/erz351⟩ |
| ISSN: | 0022-0957 1460-2431 |
| DOI: | 10.1093/jxb/erz351⟩ |
| Popis: | Oxidation of proline in response to dark-induced senescence in Arabidopsis leaves allows this amino acid to be used as an alternative respiratory substrate and contributes to the production of glutamate and energy that can be used for nutrient remobilization. Leaf senescence is a form of developmentally programmed cell death that allows the remobilization of nutrients and cellular materials from leaves to sink tissues and organs. Among the catabolic reactions that occur upon senescence, little is known about the role of proline catabolism. In this study, the involvement in dark-induced senescence of proline dehydrogenases (ProDHs), which catalyse the first and rate-limiting step of proline oxidation in mitochondria, was investigated using prodh single- and double-mutants with the help of biochemical, proteomic, and metabolomic approaches. The presence of ProDH2 in mitochondria was confirmed by mass spectrometry and immunogold labelling in dark-induced leaves of Arabidopsis. The prodh1 prodh2 mutant exhibited enhanced levels of most tricarboxylic acid cycle intermediates and free amino acids, demonstrating a role of ProDH in mitochondrial metabolism. We also found evidence of the involvement and the importance of ProDH in respiration, with proline as an alternative substrate, and in remobilization of proline during senescence to generate glutamate and energy that can then be exported to sink tissues and organs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|