The first Conus genome assembly reveals a primary genetic central dogma of conopeptides in C. betulinus
| Autor: | Jia Li, Chong-Xu Fan, Yanping Li, Chao Bian, Xinxin You, Xueqiang Lin, Ruobo Gu, Yunyun Lv, Bingmiao Gao, Jiaan Yang, Ji-Sheng Chen, Kai Zhang, Qiong Shi, Chao Peng, Junmin Xu, Jieming Chen, Yanbin He, Ge Yao, Hui Jiang, Jie Liu, Xinhui Zhang, Zhiqiang Ruan, Yu Huang, Lin Zhilong, Yunhai Yi |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
biology lcsh:Cytology Comparative genomics Proteomic analysis Sequence assembly Genomics Cell Biology Computational biology biology.organism_classification Biochemistry Genome Article Cone snail Transcriptome 03 medical and health sciences 0302 clinical medicine Conus Proteome Genetics lcsh:QH573-671 Molecular Biology Gene 030217 neurology & neurosurgery 030304 developmental biology |
| Zdroj: | Cell Discovery, Vol 7, Iss 1, Pp 1-14 (2021) Cell Discovery |
| ISSN: | 2056-5968 |
| Popis: | Although there are various Conus species with publicly available transcriptome and proteome data, no genome assembly has been reported yet. Here, using Chinese tubular cone snail (C. betulinus) as a representative, we sequenced and assembled the first Conus genome with original identification of 133 genome-widely distributed conopeptide genes. After integration of our genomics, transcriptomics, and peptidomics data in the same species, we established a primary genetic central dogma of diverse conopeptides, assuming a rough number ratio of ~1:1:1:10s for the total genes: transcripts: proteins: post-translationally modified peptides. This ratio may be special for this worm-hunting Conus species, due to the high diversity of various Conus genomes and the big number ranges of conopeptide genes, transcripts, and peptides in previous reports of diverse Conus species. Only a fraction (45.9%) of the identified conotopeptide genes from our achieved genome assembly are transcribed with transcriptomic evidence, and few genes individually correspond to multiple transcripts possibly due to intraspecies or mutation-based variances. Variable peptide processing at the proteomic level, generating a big diversity of venom conopeptides with alternative cleavage sites, post-translational modifications, and N-/C-terminal truncations, may explain how the 133 genes and ~123 transcripts can generate thousands of conopeptides in the venom of individual C. betulinus. We also predicted many conopeptides with high stereostructural similarities to the putative analgesic ω-MVIIA, addiction therapy AuIB and insecticide ImI, suggesting that our current genome assembly for C. betulinus is a valuable genetic resource for high-throughput prediction and development of potential pharmaceuticals. |
| Databáze: | OpenAIRE |
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