Engineering bacterial symbionts of nematodes improves their biocontrol potential to counter the western corn rootworm
| Autor: | Helge B. Bode, Yi-Ming Shi, Carla C. M. Arce, Evangelia Vogiatzaki, Olivier P. Schaeren, Rémy Bruggmann, Siegfried Hapfelmeier, Lisa Thönen, Daniel Wüthrich, Matthias Erb, Christelle A. M. Robert, Fausto Prada, Ricardo A. R. Machado, Matheus D. Notter, Vanitha Theepan |
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| Rok vydání: | 2019 |
| Předmět: |
animal structures
Nematoda Biomedical Engineering Biological pest control Bioengineering Agricultural pest Aquaporins Applied Microbiology and Biotechnology Zea mays Evolution Molecular 03 medical and health sciences 0302 clinical medicine Bacterial Proteins Botany Drug Resistance Bacterial Animals Pest Control Biological 030304 developmental biology Plant Diseases 0303 health sciences Larva Experimental evolution biology business.industry fungi Pest control food and beverages biochemical phenomena metabolism and nutrition biology.organism_classification Benzoxazines Western corn rootworm Mutation Molecular Medicine PEST analysis business Genetic Engineering Photorhabdus 030217 neurology & neurosurgery Biotechnology |
| Zdroj: | Nature biotechnology. 38(5) |
| ISSN: | 1546-1696 |
| Popis: | The western corn rootworm (WCR) decimates maize crops worldwide. One potential way to control this pest is treatment with entomopathogenic nematodes (EPNs) that harbor bacterial symbionts that are pathogenic to insects. However, WCR larvae sequester benzoxazinoid secondary metabolites that are produced by maize and use them to increase their resistance to the nematodes and their symbionts. Here we report that experimental evolution and selection for bacterial symbionts that are resistant to benzoxazinoids improve the ability of a nematode-symbiont pair to kill WCR larvae. We isolated five Photorhabdus symbionts from different nematodes and increased their benzoxazinoid resistance through experimental evolution. Benzoxazinoid resistance evolved through multiple mechanisms, including a mutation in the aquaporin-like channel gene aqpZ. We reintroduced benzoxazinoid-resistant Photorhabdus strains into their original EPN hosts and identified one nematode-symbiont pair that was able to kill benzoxazinoid-sequestering WCR larvae more efficiently. Our results suggest that modification of bacterial symbionts might provide a generalizable strategy to improve biocontrol of agricultural pests. |
| Databáze: | OpenAIRE |
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