Expression of sulfotransferases involved in the biosynthesis of chondroitin sulfate E in the bone marrow derived mast cells
| Autor: | Osami Habuchi, Sachiko Kondo, Toshiko Morisaki, Shiori Ohtake, Koji Kimata, Kaori Matsumura |
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| Rok vydání: | 2008 |
| Předmět: |
Sulfotransferase
Molecular Sequence Data Biophysics Bone Marrow Cells sulfotransferase Disaccharides Biochemistry Ammonium Chloride Gene Expression Regulation Enzymologic law.invention Mice chemistry.chemical_compound Sulfation Biosynthesis law medicine bone marrow derived mast cells Animals Chondroitin Mast Cells Chondroitin sulfate Molecular Biology Cells Cultured Chromatography High Pressure Liquid Mice Inbred BALB C Dose-Response Relationship Drug Reverse Transcriptase Polymerase Chain Reaction Chemistry Chondroitin Sulfates chondroitin sulfate E Chloroquine Hydrogen-Ion Concentration Recombinant Proteins carbohydrates (lipids) medicine.anatomical_structure bafilomycin A Chromatography Gel Recombinant DNA Macrolides Bone marrow Sulfotransferases GalNAc4S-6ST Intracellular |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - General Subjects. 1780:687-695 |
| ISSN: | 0304-4165 |
| DOI: | 10.1016/j.bbagen.2008.01.004 |
| Popis: | Bone marrow-derived mast cells (BMMCs) contain chondroitin sulfate (CS)-E comprised of GlcA-GalNAc(4SO_4) units and GlcA-GalNAc(4,6-SO_4) units. GalNAc 4-sulfate 6-O-sulfotransferase (GalNAc4S-6ST) transfers sulfate to position 6 of GalNAc(4SO_4) residues of CS. On the basis of the specificity of GalNAc4S-6ST, it is thought that CS-E is synthesized in BMMC through the sequential sulfation by chondroitin 4-sulfotransferase (C4ST)-1 and GalNAc4S-6ST. In this paper, we investigated whether GalNAc4S-6ST and C4ST-1 are actually expressed in BMMCs in which CS-E is actively synthesized. As the bone marrow cells differentiate to BMMCs, level of C4ST-1 and GalNAc4S-6ST messages increased, whereas chondroitin 6-sulfotransferase (C6ST)-1 message decreased. In the extract of BMMCs, activity of GalNAc4S-6ST and C4ST but not C6ST were detected. The recombinant mouse GalNAc4S-6ST transferred sulfate to both nonreducing terminal and internal GalNAc(4SO_4) residues; the activity toward nonreducing terminal GalNAc(4SO_4) was increased with increasing pH. When CS-E synthesized by BMMCs was metabolically labeled with ^SO_4 in the presence of bafilomycin A, chloroquine or NH_4Cl, the proportion of the nonreducing terminal GalNAc(4,6-SO_4) was increased compared with the control, suggesting that GalNAc4S-6ST in BMMC may elaborate CS-E in the intracellular compartment with relatively low pH where sulfation of the internal GalNAc(4SO_4) by GalNAc4S-6ST preferentially occurs. |
| Databáze: | OpenAIRE |
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