Immunogenicity and protection efficacy evaluation of avian paramyxovirus serotype-1 (APMV-1) isolates in experimentally infected chickens
| Autor: | Kizito K. Mugimba, Denis K. Byarugaba, Maxwell Otim-Onapa, Halid Kirunda, John Bosco Omony, Agnes Wanyana, Jessica L. Nakavuma |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Serotype animal structures 040301 veterinary sciences Newcastle Disease Newcastle disease virus Chick Embryo Virus Replication Newcastle disease Microbiology 0403 veterinary science 03 medical and health sciences Immunogenicity Vaccine Food Animals Antigen Animals Poultry Diseases General Immunology and Microbiology biology Base Sequence Virulence Reverse Transcriptase Polymerase Chain Reaction Immunogenicity Viral Vaccine Outbreak Viral Vaccines 04 agricultural and veterinary sciences biology.organism_classification Virology Vaccination 030104 developmental biology DNA Viral biology.protein RNA Viral Animal Science and Zoology Antibody Chickens |
| Zdroj: | Avian pathology : journal of the W.V.P.A. 46(4) |
| ISSN: | 1465-3338 |
| Popis: | Vaccine failures after Newcastle disease vaccination with the current commercial vaccines have been reported and are associated with many factors, including genotypic and antigenic differences between vaccine and outbreak strains, although all APMV-1 members belong to one serotype. We assessed the immunoprotection ability of four thermostable, low-virulent Newcastle disease-virus isolates from Ugandan waterfowl against challenge with a virulent strain (MDT = 36.8 h, ICPI = 1.78) isolated from morbid chicken. Six-week-old commercial Leghorn layers, challenged at 21 days post immunization were used. Four isolates designated: NDV-133/UG/MU/2011, NDV-177/UG/MU/2011, NDV-178/UG/MU/2011 and NDV-173/UG/MU/2011 induced mean haemagglutinin inhibition antibody titres of log2 9.3, 8.2, 6.3 and 2.0, respectively, at 21 days post immunization. The antibody titres correlated with the protection rates (R² = 0.86, p |
| Databáze: | OpenAIRE |
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