Determination of human serum semicarbazide-sensitive amine oxidase activity via flow injection analysis with fluorescence detection after online derivatization of the enzymatically produced benzaldehyde with 1,2-diaminoanthraquinone
| Autor: | Takahiro Imazato, Mahmoud El-Maghrabey, Yukitaka Ueki, Kaname Ohyama, Naotaka Kuroda, Naoya Kishikawa |
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| Rok vydání: | 2015 |
| Předmět: |
Semicarbazide-sensitive amine oxidase activity
Male Benzylamines Amine oxidase Anthraquinones Biochemistry Fluorescence detection Substrate Specificity Analytical Chemistry Benzaldehyde chemistry.chemical_compound Benzylamine Limit of Detection Diabetes Mellitus Humans Environmental Chemistry Derivatization Spectroscopy Detection limit Flow injection analysis Chromatography Molecular Structure 1 2-Diaminoanthraquinone Chemistry Imidazoles Reproducibility of Results Middle Aged Fluorescence Spectrometry Fluorescence Benzaldehydes Reagent Flow Injection Analysis Female Amine Oxidase (Copper-Containing) |
| Zdroj: | Analytica Chimica Acta. 881:139-147 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/j.aca.2015.04.006 |
| Popis: | A fast, simple, and sensitive flow injection analysis method was developed for the measurement of semicarbazide-sensitive amine oxidase (SSAO) activity in human serum. Benzaldehyde, generated by the action of SSAO after incubation of serum with benzylamine, was derivatized with a novel aromatic aldehyde-specific reagent (1,2-diaminoanthraquinone) and the fluorescent product was measured by fluorescence detection at excitation and emission wavelengths of 390 and 570nm, respectively. Serum SSAO activity was defined as benzaldehyde (nmol) formed per milliliter serum per hour. The method was linear over SSAO activity of 0.2-150.0nmolmL-1h-1 with a detection limit of 0.06nmolmL-1h-1. The %RSD of intra-day and inter-day precision did not exceed 9.4% and the accuracy ranged from -6.5 to -0.6%. The method was applied for the determination of the serum SSAO activity in healthy controls (C, n=24) and diabetes mellitus patients (DM, n=18). It was demonstrated that the activity (mean±SE) of SSAO in diabetics sera was significantly higher than that in healthy subjects' ones (DM; 73.3±1.8nmolmL-1h-1 vs C; 58.9±2.2nmolmL-1h-1, P Analytica Chimica Acta, 881, pp.139-147; 2015 |
| Databáze: | OpenAIRE |
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