Atherin: a newly identified, lesion-specific, LDL-binding protein in human atherosclerosis
| Autor: | Bruce D. Campbell, Ann M. Lees, Robert S. Lees, Anne E. Deconinck |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Pathology
medicine.medical_specialty Molecular Sequence Data Vesicular Transport Proteins Aorta Thoracic Biology Actin-Related Protein 2-3 Complex Antibodies Pathogenesis Lesion medicine Extracellular Animals Humans Amino Acid Sequence Aorta Abdominal Gene Library Vascular disease Binding protein Macrophages Cholesterol LDL medicine.disease Atherosclerosis Immunohistochemistry medicine.anatomical_structure Receptors LDL Circulatory system lipids (amino acids peptides and proteins) Rabbits medicine.symptom Cardiology and Cardiovascular Medicine Blood vessel Artery Foam Cells |
| Zdroj: | Atherosclerosis. 182(2) |
| ISSN: | 0021-9150 |
| Popis: | Prolonged retention of LDL in focal, atherosclerosis-prone areas of arteries is a primary event in atherogenesis. To determine whether unrecognized LDL-binding proteins participate in this process, we generated a cDNA expression library from deendothelialized rabbit aorta, a model for early atherosclerosis that shows striking focal LDL retention in healing lesions. Library screening identified a previously unknown, highly conserved, 56 kDa LDL-binding protein that we call atherin. Confocal microscopy of human arteries shows that atherin is present only in atherosclerotic lesions, not in normal intima. Within lesions, atherin is found both in the extracellular compartment and within foam cells. Essentially all extracellular atherin, as well as atherin within foam cells, co-localizes with LDL across the entire spectrum of human disease, from early lesions to advanced plaques. Our results suggest that focal arterial LDL accumulation may be initiated and maintained by binding between LDL and atherin, and that atherin may play a central role in atherogenesis by immobilizing LDL in the arterial wall. |
| Databáze: | OpenAIRE |
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