Human Toll-like receptor 2 mediates induction of the antimicrobial peptide human beta-defensin 2 in response to bacterial lipoprotein
| Autor: | Reinhart Seibl, Roger Lauener, Susanne Loeliger, Johann Peter Hossle, Thomas Birchler, Katja Büchner, Reinhard Seger, Adriano Aguzzi |
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| Přispěvatelé: | University of Zurich, Lauener, R P |
| Rok vydání: | 2001 |
| Předmět: |
beta-Defensins
Transcription Genetic Lipoproteins Immunology Antimicrobial peptides 10208 Institute of Neuropathology 610 Medicine & health Receptors Cell Surface Biology Microbiology Cell Line Bacterial Proteins Immunology and Allergy Drosophila Proteins Humans Receptor A549 cell 2403 Immunology Toll-like receptor Innate immune system Membrane Glycoproteins fungi HEK 293 cells Toll-Like Receptors NF-kappa B Toll-Like Receptor 2 Beta defensin Interleukin-1 Receptor-Associated Kinases 2723 Immunology and Allergy 570 Life sciences biology Signal transduction Protein Kinases |
| Zdroj: | European journal of immunology. 31(11) |
| ISSN: | 0014-2980 |
| Popis: | Recognition of pathogens by Drosophila Toll or human Toll-like receptors results in translocation of Dorsal or its human homologue NF-kappaB, respectively; in Drosophila, this is followed by the production of antimicrobial peptides serving as antimicrobial effector system of the innate immune response. We investigated whether human Toll-like receptors also mediate induction of the synthesis of antimicrobial peptides. We found that HEK293 cells transfected with Toll-like receptor 2, but not wild-type cells responded to stimulation with bacterial lipoprotein by production of human beta-defensin 2. Furthermore, the human lung epithelial cell line A549 was found to constitutively express Toll-like receptor 2 and to produce beta-defensin 2 in response to bacterial lipoprotein. This response was abrogated by blocking the signaling pathway activated through Toll-like receptors by transfecting the A549 cells with a dominant-negative form of IRAK-2. Thus, exposure of human cells to bacterial lipoprotein elicits production of the antimicrobial peptide beta-defensin 2 through Toll-like receptor 2. |
| Databáze: | OpenAIRE |
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