Regulation of C2C12 myogenic terminal differentiation by MKK3/p38α pathway
| Autor: | Walter Englaro, Michel Ragno, Candice Cabane, Benoit Dérijard, Karen Yeow |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
MAPK/ERK pathway
Myosin Light Chains Physiology MAP Kinase Kinase 3 Myoblasts Skeletal Cellular differentiation Muscle Fibers Skeletal MAP Kinase Kinase Kinase 1 Cell Cycle Proteins Protein Serine-Threonine Kinases MyoD Mitogen-Activated Protein Kinase 14 Mice MyoD Protein Animals Muscle Skeletal Cells Cultured Myogenin Mitogen-Activated Protein Kinase Kinases Myosin Heavy Chains biology Myogenesis Muscle cell differentiation Tumor Suppressor Proteins Cell Differentiation Cell Biology Protein-Tyrosine Kinases Cell biology Biochemistry Mitogen-activated protein kinase biology.protein Mitogen-Activated Protein Kinases Cyclin-Dependent Kinase Inhibitor p27 |
| Zdroj: | American Journal of Physiology-Cell Physiology. 284:C658-C666 |
| ISSN: | 1522-1563 0363-6143 |
| DOI: | 10.1152/ajpcell.00078.2002 |
| Popis: | The signal transduction pathways connecting cell surface receptors to the activation of muscle-specific promoters and leading to myogenesis are still largely unknown. Recently, a contribution of the p38 mitogen-activated protein kinase (MAPK) pathway to this process was evoked through the use of pharmacological inhibitors. We used several mutants of the kinases composing this pathway to modulate the activity of the muscle-specific myosin light chain and myogenin promoters in C2C12 cells by transient transfections. In addition, we show for the first time, using a stable C2C12 cell line expressing a dominant-negative form of the p38 activator MAPK kinase (MKK)3, that a functional p38 MAPK pathway is indeed required for terminal muscle cell differentiation. The most obvious phenotype of this cell line, besides the inhibition of the activation of p38, is its inability to undergo terminal differentiation. This phenotype is accompanied by a drastic inhibition of cell cycle and myogenesis markers such as p21, p27, MyoD, and troponin T, as well as a profound disorganization of the cytoskeleton. |
| Databáze: | OpenAIRE |
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