Long-term in vitro culture of Plasmodium vivax isolates from Madagascar maintained in Saimiri boliviensis blood
| Autor: | Melinda Zikursh-Blood, Marlin Linger, Arsène Ratsimbasoa, Rosalind E. Howes, Peter A. Zimmerman, Brune Ramiranirina, Tovonahary Angelo Rakotomanga, Rajeev K. Mehlotra, Stéphanie Ramboarina, D’Arbra Blankenship, Thierry Franchard, Brian T. Grimberg |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
lcsh:Arctic medicine. Tropical medicine Erythrocytes lcsh:RC955-962 Plasmodium vivax Cell Culture Techniques lcsh:Infectious and parasitic diseases Specimen Handling 03 medical and health sciences parasitic diseases Madagascar medicine Animals Humans Parasite hosting lcsh:RC109-216 Saimiri Cryopreservation biology Research Tropical disease Plasmodium falciparum In vitro culture biology.organism_classification medicine.disease Virology 3. Good health 030104 developmental biology Infectious Diseases Saimiri boliviensis Parasitology Sample collection Malaria |
| Zdroj: | Malaria Journal Malaria Journal, Vol 16, Iss 1, Pp 1-13 (2017) |
| Popis: | Background Plasmodium vivax is the most prevalent human malaria parasite and is likely to increase proportionally as malaria control efforts more rapidly impact the prevalence of Plasmodium falciparum. Despite the prominence of P. vivax as a major human pathogen, vivax malaria qualifies as a neglected and under-studied tropical disease. Significant challenges bringing P. vivax into the laboratory, particularly the capacity for long-term propagation of well-characterized strains, have limited the study of this parasite’s red blood cell (RBC) invasion mechanism, blood-stage development, gene expression, and genetic manipulation. Methods and results Patient isolates of P. vivax have been collected and cryopreserved in the rural community of Ampasimpotsy, located in the Tsiroanomandidy Health District of Madagascar. Periodic, monthly overland transport of these cryopreserved isolates to the country’s National Malaria Control Programme laboratory in Antananarivo preceded onward sample transfer to laboratories at Case Western Reserve University, USA. There, the P. vivax isolates have been cultured through propagation in the RBCs of Saimiri boliviensis. For the four patient isolates studied to-date, the median time interval between sample collection and in vitro culture has been 454 days (range 166–961 days). The median time in culture, continually documented by light microscopy, has been 159 days; isolate AMP2014.01 was continuously propagated for 233 days. Further studies show that the P. vivax parasites propagated in Saimiri RBCs retain their ability to invade human RBCs, and can be cryopreserved, thawed and successfully returned to productive in vitro culture. Conclusions/significance Long-term culture of P. vivax is possible in the RBCs of Saimiri boliviensis. These studies provide an alternative to propagation of P. vivax in live animals that are becoming more restricted. In vitro culture of P. vivax in Saimiri RBCs provides an opening to stabilize patient isolates, which would serve as precious resources to apply new strategies for investigating the molecular and cellular biology of this important malaria parasite. Electronic supplementary material The online version of this article (10.1186/s12936-017-2090-7) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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