Optimization of Formulations Consisting of Layered Double Hydroxide Nanoparticles and Small Interfering RNA for Efficient Knockdown of the Target Gene
| Autor: | Son Trong Do, Yanheng Wu, Chen Chen, Wenyi Gu, Zhi Ping Xu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Gene knockdown
Small interfering RNA Chemistry General Chemical Engineering 02 engineering and technology General Chemistry Gene delivery 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences In vitro Article 0104 chemical sciences lcsh:Chemistry chemistry.chemical_compound lcsh:QD1-999 In vivo Biophysics Hydroxide Gene silencing 0210 nano-technology Gene |
| Zdroj: | ACS Omega ACS Omega, Vol 3, Iss 5, Pp 4871-4877 (2018) |
| ISSN: | 2470-1343 |
| Popis: | Layered double hydroxide (LDH) nanoparticles (NPs) are safe and effective vectors for small interfering RNA (siRNA) delivery. However, it is unclear whether there are optimal parameters for the efficient delivery of functional siRNA using LDH NPs. In this research, we comprehensively examined the effect of parameters, such as the mixing method and LDH/siRNA mass ratio on siRNA silencing capability. We first noted that the best way for loading gene segments (25 bp dsDNA and siRNA) is to add gene molecules to 100 nm LDH and then diluting in Dulbecco's modified Eagle's medium. Very interestingly, the optimal LDH/gene mass ratio is around 20:1 in terms of cellular uptake amount of gene segments, whereas this ratio is shifted to around 5:1 in terms of target gene silencing efficacy, which has been reasonably explained. The optimization for LDH NP-based gene delivery system may provide the guidance for more efficient in vitro and in vivo siRNA delivery using the optimal parameters. |
| Databáze: | OpenAIRE |
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