Optical model of the murine lung to optimize pulmonary illumination
| Autor: | Fatemeh Nooshabadi, Kristen C. Maitland, Jeffrey D. Cirillo, Madeleine S. Durkee |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Dorsum Optical fiber Materials science education Monte Carlo method Biomedical Engineering Models Biological 01 natural sciences Imaging phantom law.invention 010309 optics Biomaterials Mice 03 medical and health sciences law 0103 physical sciences medicine Animals Tuberculosis Whole Body Imaging Mouse Lung Lung Phantoms Imaging Optical Imaging Mycobacterium tuberculosis Torso Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials 030104 developmental biology medicine.anatomical_structure Murine lung Special Section on Selected Topics in Biophotonics: Optogenetics and Label-Free Optical Spectroscopy Fluence rate Biomedical engineering |
| Zdroj: | J Biomed Opt |
| ISSN: | 1083-3668 |
| DOI: | 10.1117/1.jbo.23.7.071208 |
| Popis: | We describe a Monte Carlo model of the mouse torso to optimize illumination of the mouse lung for fluorescence detection of low levels of pulmonary pathogens, specifically Mycobacterium tuberculosis. After validation of the simulation with an internally illuminated optical phantom, the entire mouse torso was simulated to compare external and internal illumination techniques. Measured optical properties of deflated mouse lungs were scaled to mimic the diffusive properties of inflated lungs in vivo. Using the full-torso model, a [Formula: see text] to [Formula: see text] improvement in average fluence rate in the lung was seen for dorsal compared with ventral positioning of the mouse with external illumination. The enhancement in average fluence rate in the lung using internal excitation was [Formula: see text] to [Formula: see text] over external illumination in the dorsal position. Parameters of the internal fiber optic source were manipulated in the model to guide optimization of the physical system and experimental protocol for internal illumination and whole-body detection of fluorescent mycobacteria in a mouse model of infection. |
| Databáze: | OpenAIRE |
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