Novel regulation targets of the metal-response BasS–BasR two-component system of Escherichia coli
| Autor: | Kaneyoshi Yamamoto, Shota Shinohara, Hiroshi Ogasawara, Akira Ishihama |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Regulation of gene expression
Genetics Binding Sites Base Sequence Transcription Genetic Escherichia coli Proteins Molecular Sequence Data Gene Expression Regulation Bacterial Biology medicine.disease_cause Microbiology Genome Two-component regulatory system Cell biology Regulon Metals Escherichia coli medicine Direct repeat Gene Systematic evolution of ligands by exponential enrichment Protein Binding |
| Zdroj: | Microbiology. 158:1482-1492 |
| ISSN: | 1465-2080 1350-0872 |
| DOI: | 10.1099/mic.0.057745-0 |
| Popis: | The BasS-BasR two-component system is known as an iron- and zinc-sensing transcription regulator in Escherichia coli, but so far only a few genes have been identified to be under the direct control of phosphorylated BasR. Using Genomic SELEX (systematic evolution of ligands by exponential enrichment) screening, we have identified a total of at least 38 binding sites of phosphorylated BasR on the E. coli genome, and based on the BasR-binding sites, have predicted more than 20 novel targets of regulation. By DNase I footprint analysis for high-affinity BasR-binding sites, a direct repeat of a TTAAnnTT sequence was identified as the BasR box. Transcription regulation in vivo of the target genes was confirmed after Northern blot analysis of target gene mRNAs from both wild-type E. coli and an otherwise isogenic basR deletion mutant. The BasR regulon can be classified into three groups of genes: group 1 includes the genes for the formation and modification of membrane structure; group 2 includes genes for modulation of membrane functions; and group 3 includes genes for stress-response cell functions, including csgD, the master regulator of biofilm formation. |
| Databáze: | OpenAIRE |
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