Knockdown of CSE1L Gene in Colorectal Cancer Reduces Tumorigenesis in Vitro
| Autor: | Dung Tsa Chen, Kevin G Neill, Kazim Husain, Steven A. Eschrich, Evita Henderson-Jackson, Mokenge P. Malafa, Jose M. Pimiento, Domenico Coppola, David Shibata |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Adenoma Adult Male Cytoplasm Carcinogenesis Apoptosis Biology medicine.disease_cause Pathology and Forensic Medicine 03 medical and health sciences Young Adult 0302 clinical medicine Cellular Apoptosis Susceptibility Protein Cell Line Tumor medicine Humans neoplasms Cellular localization Aged Cell Proliferation Regulation of gene expression Aged 80 and over Cell Nucleus Gene knockdown Tissue microarray Epithelial Cells Regular Article Middle Aged Molecular biology digestive system diseases 3. Good health Gene Expression Regulation Neoplastic Protein Transport 030104 developmental biology Tissue Array Analysis 030220 oncology & carcinogenesis Immunohistochemistry Female Colorectal Neoplasms Cellular apoptosis susceptibility protein |
| Zdroj: | The American journal of pathology. 186(10) |
| ISSN: | 1525-2191 |
| Popis: | Human cellular apoptosis susceptibility (chromosomal segregation 1-like, CSE1L) gene plays a role in nuclear-to-cytoplasm transport and chromosome segregation during mitosis, cellular proliferation, and apoptosis. CSE1L is involved in colon carcinogenesis. CSE1L gene expression was assessed with three data sets using Affymetrix U133 + gene chips on normal human colonic mucosa (NR), adenomas (ADs), and colorectal carcinoma (CRC). CSE1L protein expression in CRC, AD, and NR from the same patients was measured by immunohistochemistry using a tissue microarray. We evaluated CSE1L expression in CRC cells (HCT116, SW480, and HT29) and its biological functions. CSE1L mRNA was significantly increased in all AD and CRC compared with NR (P |
| Databáze: | OpenAIRE |
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