Can soil properties and agricultural land use affect arbuscular mycorrhizal fungal communities indigenous from the Argentinean Pampas soils?
| Autor: | H. Sainz Rozas, A.J. Thougnon Islas, Hernán Eduardo Echeverría, K. Hernandez Guijarro, Fernanda Covacevich, Mercedes Eyherabide |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
Soil biology Soil Science Mycorriza 01 natural sciences Ciencias Biológicas Soil Botany Colonization Mycorrhiza Glomus Ecology biology fungi Spore Edaphic 04 agricultural and veterinary sciences DNA Bioquímica y Biología Molecular biology.organism_classification Agricultural and Biological Sciences (miscellaneous) SSCP Glomalin Arbuscular mycorrhiza Soil water Land use 040103 agronomy & agriculture biology.protein 0401 agriculture forestry and fisheries CIENCIAS NATURALES Y EXACTAS 010606 plant biology & botany |
| Popis: | In order to determine if intensive agricultural land use and/or edaphic characteristics modify thecommunity of the arbuscular mycorrhizal fungi (AMF) indigenous of soils from the Argentinean Pampa;mycorrhizal colonization, sporulation, glomalin (GRSP) and genetic diversity were assessed. Soils werecollected from seven localities from Buenos Aires Province (Argentina) under contrasting land useintensity (at each site both pristine and agricultural soils). The capacity for root colonization, sporulationand glomalin content of trap plants and growth substrate were considered as measure of AMF activity.Analysis of diversity was conducted using PCR-single strand conformation polymorphism (SSCP). Profileswere generated by using primers to amplify the 28S rDNA of AMF from rDNA extracted directly from thesubstrate of trap plant or AMF spore. Five bands of the SSCP gel were sequenced and showed similaritywith the genus Glomus. Analysis of colonization and GRSP content, were not clearly related to the land useintensity but sporulation capacity was, in general, lower in agricultural soils than in pristine ones. Soilphosphorus, ferrum and zinc contents, were negatively related to the root colonization and sporulationcapacities. High genetic diversity was found both from soil and spore samples. Although similar diversitywas found under agricultural and pristine soils, in most sites SSCP-diversity was separately clustered byland use. Still remains clarify if soil characteristics, resulting from agricultural management; exertselection pressure on the AMF and whether they could be vectors of interest to select potential plantgrowth promoting microorganisms. Fil: Thougnon Islas, Andrea Julieta. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hernandez Guijarro, K.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina Fil: Eyherabide, Mercedes. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Area de Invest.en Agronomia; Argentina Fil: Sainz Rozas, Hernan Rene. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Area de Invest.en Agronomia; Argentina Fil: Echeverria, Hernan Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Covacevich, Fernanda. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones En Biodiversidad y Biotecnología; Argentina |
| Databáze: | OpenAIRE |
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