Increased nitric oxide availability worsens the cardiac performance during early re-perfusion period in adult rats
| Autor: | Faten M. A. Diab, Enas A. Abdel-Hady, Mahmoud H. Ayobe, Mohamed F. Abdel-Salam, Mohammed F S Otman |
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| Rok vydání: | 2020 |
| Předmět: |
Chronotropic
Inotrope medicine.medical_specialty Physiology Diastole Ischemia 030204 cardiovascular system & hematology Nitric oxide 03 medical and health sciences Basal (phylogenetics) chemistry.chemical_compound 0302 clinical medicine Internal medicine Drug Discovery medicine Myocardial infarction 030304 developmental biology Pharmacology 0303 health sciences business.industry General Medicine medicine.disease chemistry Cardiology business Perfusion |
| Zdroj: | Journal of basic and clinical physiology and pharmacologyReferences. |
| ISSN: | 2191-0286 2020-0358 |
| Popis: | Objectives Re-perfusion is the standard therapy for acute myocardial infarction, despite the associated pathologies that may contribute to irreversible myocardial injury. The present study aims to clarify the alterations in cardiac activities in response to experimental cardiac ischemic arrest followed by re-perfusion in isolated hearts perfused with nitric oxide (NO) donor, l-arginine, or NO inhibitor, Nω-Nitro-l-arginine methyl ester hydrochloride (l-NAME), to shed light on the possible role of NO in the re-perfusion process. Methods Hearts isolated from adult Wistar rats were studied on Langendorff preparation under basal conditions and during 30 min re-perfusion following 30 min of total global ischemia. Rats were randomly divided into three groups; control and l-arginine or l-NAME infused heart groups. Cardiac tissue content of malondialdhyde, catalase and nitrite was also measured. Results Compared to the control group, both l-arginine and l-NAME infused hearts showed increased basal chronotropy and myocardial flow rate. Following ischemia and during the whole period of re-perfusion, the three groups demonstrated significant deterioration in the inotropic activity and compromised myocardial flow rate. l-arginine infused hearts revealed depressed inotropy and chronotropy, weak systolic and diastolic functions with compromised myocardial flow at early 5 min of re-perfusion, yet with significantly higher myocardial flow rate by the end of re-perfusion. Conclusions Reducing NO availability by l-NAME revealed mild impact on the ischemia re-perfusion induced contractile dysfunction, whereas excess NO worsens cardiac performance at the early re-perfusion period. |
| Databáze: | OpenAIRE |
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