Polyethylene imine/graphene oxide layer-by-layer surface functionalization for significantly improved limit of detection and binding kinetics of immunoassays on acrylate surfaces
| Autor: | Marystela Ferreira, Rohit Mishra, Celina M. Miyazaki, David J. Kinahan, Jens Ducrée |
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| Rok vydání: | 2017 |
| Předmět: |
Materials science
Enzyme-Linked Immunosorbent Assay macromolecular substances 02 engineering and technology 010402 general chemistry 01 natural sciences law.invention Contact angle chemistry.chemical_compound Colloid and Surface Chemistry Adsorption law Polymer chemistry Polyethyleneimine Polymethyl Methacrylate Physical and Theoretical Chemistry Immunoassay Acrylate Graphene Layer by layer technology industry and agriculture Surfaces and Interfaces General Medicine 021001 nanoscience & nanotechnology 0104 chemical sciences Kinetics chemistry Chemical engineering Surface modification Graphite Polystyrene Wetting 0210 nano-technology Biotechnology |
| Zdroj: | Colloids and surfaces. B, Biointerfaces. 158 |
| ISSN: | 1873-4367 |
| Popis: | Antibody immobilization on polymeric substrates is a key manufacturing step for microfluidic devices that implement sample-to-answer automation of immunoassays. In this work, a simple and versatile method to bio-functionalize poly(methylmethacrylate) (PMMA), a common material of such "Lab-on-a-Chip" systems, is proposed; using the Layer-by-Layer (LbL) technique, we assemble nanostructured thin films of poly(ethylene imine) (PEI) and graphene oxide (GO). The wettability of PMMA surfaces was significantly augmented by the surface treatment with (PEI/GO)5 film, with an 81% reduction of the contact angle, while the surface roughness increased by 600%, thus clearly enhancing wettability and antibody binding capacity. When applied to enzyme-linked immunosorbent assays (ELISAs), the limit of detection of PMMA surface was notably improved from 340pgmL-1 on commercial grade polystyrene (PS) and 230pgmL-1 on plain PMMA surfaces to 130pgmL-1 on (PEI/GO)5 treated PMMA. Furthermore, the accelerated antibody adsorption kinetics on the LbL films of GO allowed to substantially shorten incubation times, e.g. for anti-rat IgG adsorption from 2h down to 15min on conventional and treated surfaces, respectively. |
| Databáze: | OpenAIRE |
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