The unique endometrial expression and genomic organization of the porcine IGFBP-2 gene
| Autor: | Sihong Song, Frank A. Simmen, Rosalia C. M. Simmen, Chung S. Chung, C. Young Lee, Michael L. Green |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Swine
Molecular Sequence Data Gene Expression Biology Endometrium Biochemistry Exon Endocrinology Pregnancy Gene expression medicine Animals Amino Acid Sequence Molecular Biology Gene Messenger RNA Genome Base Sequence Myometrium Molecular biology Insulin-Like Growth Factor Binding Protein 2 medicine.anatomical_structure CpG site Organ Specificity Female Sequence Analysis hormones hormone substitutes and hormone antagonists Immunostaining |
| Zdroj: | Molecular and Cellular Endocrinology. 120:193-202 |
| ISSN: | 0303-7207 |
| DOI: | 10.1016/0303-7207(96)03837-3 |
| Popis: | The insulin-like growth factor-binding proteins (IGFBPs-1-6) modulate the mitogenic and differentiative actions of the IGFs and may have IGF-independent functions. This study examined the gene expression and pregnancy-regulation of the IGF/IGFBP system in porcine uterine endometrium and myometrium during the periimplantation period and later stages of pregnancy. Endometrial IGFBP-2 mRNA abundance exhibited stage of pregnancy-dependent induction; whereas little or no IGFBP-2 mRNA was found in myometrium. IGFBP-2 protein was immunolocalized to the endometrial glandular and luminal epithelia (staining on day 60 > day 12) with minimal or no immunostaining of uterine stroma observed. IGFBP-3 and IGFBP-4 transcript levels became elevated in endometrium after implantation; whereas, IGFBP-5 and IGFBP-6 mRNAs were in greater abundance in periimplantation than post-implantation endometrium. IGFBP-1 transcripts, in contrast, could not be identified in porcine endometrium or myometrium of pregnancy. As a pre-requisite to understanding the pregnancy-induction and endometrial-specificity of the uterine-expressed IGFBP-2 gene, cosmids encompassing the pig IGFBP-2 chromosomal locus were isolated and characterized. This gene is comprised of four exons that span > 29 kb and encode a 316 amino acid precursor protein. All four exons were found to be G/C rich with exon 1 and immediate 5' flank exhibiting hallmarks of a CpG island. This latter region was devoid of TATA and CAAT motifs. Results identify the preferential endometrial expression of different IGFBP genes at either the periimplantation or post-implantation periods, perhaps reflecting distinct actions of these proteins at the embryo-maternal and feto-maternal interfaces, respectively. Interactions of steroid receptors, endometrial transcription factors and their corresponding cis elements may confer the unique uterine expression of the IGFBP-2 gene. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect