The endogenous, immunologically active peptide apelin inhibits lymphocytic cholinergic activity during immunological responses
| Autor: | Takeshi Fujii, Koichiro Kawashima, Yoko Horiuchi, Yuichiro Kamimura |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Interleukin 2
medicine.medical_specialty Leukemia T-Cell Immunology Down-Regulation Biology Cholinergic Antagonists Choline O-Acetyltransferase Receptors G-Protein-Coupled Jurkat Cells Internal medicine Cell Line Tumor medicine Leukemia B-Cell Immunology and Allergy Humans RNA Messenger Enzyme Inhibitors Receptor Apelin receptor Orphan receptor Apelin Receptors Molecular biology Choline acetyltransferase Acetylcholine Actins Lymphocyte Subsets Apelin Endocrinology Neurology Cholinergic Intercellular Signaling Peptides and Proteins Neurology (clinical) Carrier Proteins Immunosuppressive Agents medicine.drug |
| Zdroj: | Journal of neuroimmunology. 144(1-2) |
| ISSN: | 0165-5728 |
| Popis: | We investigated the effects of apelin, an immunologically active peptide ligand for orphan receptor APJ, on acetylcholine (ACh) synthesis in MOLT-3 human leukemic T cells. We initially confirmed expression of APJ mRNA in several human T- and B-cell lines by reverse transcription-polymerase chain reaction (RT-PCR). We also found that in phytohemagglutinin (PHA)-stimulated MOLT-3 cells, an active apelin fragment, apelin-13, down-regulates expression of choline acetyltransferase (ChAT) mRNA and significantly reduces ChAT activity and cellular ACh content and release. It thus appears that apelin inhibits lymphocytic cholinergic activity via APJ during immunological responses. |
| Databáze: | OpenAIRE |
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